Plant-derived squalene supplementation improves growth performance and alleviates acute oxidative stress-induced growth retardation and intestinal damage in piglets  

作　　者：Junquan Tian Qian Jiang Xuetai Bao Fan Yang Yuying Li Haihui Sun Kang Yao Yulong Yin 

机构地区：[1]National Engineering Laboratory for Pollution Control and Waste Utilization in Livestock and Poultry Production,Key Laboratory of Agro-ecological Processes in Subtropical Region,Laboratory of Animal Nutritional Physiology and Metabolic Process,Institute of Subtropical Agriculture,Chinese Academy of Sciences,Changsha,410125,China [2]University of Chinese Academy of Sciences,Beijing,100008,China [3]College of Animal Science and Technology,Hunan Agricultural University,Changsha,410000,China [4]Institute of Bast Fiber Crops,Chinese Academy of Agricultural Sciences,Changsha,410205,China [5]Yichun Dahaigui Life Science Co.,Ltd,Yichun,336000,China

出　　处：《Animal Nutrition》2023年第4期386-398,共13页动物营养（英文版）

基　　金：supported by the National Key R&D Plan of China(2021YFD1300400.2022YFD1300403);the National Natural Science Foundation of China(32130099);Taishan Industrial Experts Program(tscy20190121).

摘　　要：Piglets are particularly susceptible to oxidative stress,which causes inferior growth performance and intestinal damage.Squalene(SQ),a natural bioactive substance enriched in shark liver oil,shows excellent antioxidant properties and can currently be obtained at a low cost from deodorizer distillate during the production of plant oil.This study aimed to evaluate the effects of plant-derived SQ supplementation on the growth performance of piglets and explore the beneficial roles of SQ against oxidative stress and intestinal injury in diquat-challenged piglets.Forty piglets were randomly divided into five groups and fed a basal diet supplemented with SQ at 0,500,1000,or 2000 mg/kg for 5 weeks.Acute oxidative stress was induced in the piglets with diquat(10 mg/kg BW)at the fourth week of the experiment,followed by a 7-d recovery period.Results showed that before the diquat challenge,SQ supplementation significantly improved growth performance(average daily gain and feed conversion ratio)and serum antioxidant status,and after the diquat challenge,SQ supplementation significantly mitigated diquat-induced growth arrest,intestinal villous atrophy,intestinal epithelial cell apoptosis,intestinal hyperpermeability,and deficiency of intestinal epithelial tight junction proteins(zonula occludens-1,occludin,and claudin-3).Under oxidative stress induced by diquat,SQ supplementation consistently improved the antioxidant status of the small intestine,liver,and muscle.In vitro,SQ was shown to alleviate hydrogen peroxide(H2O2)-induced increase of the levels of intracellular reactive oxygen species and apoptosis of porcine intestinal epithelial cells.Taken together,SQ supplementation improves growth performance and effectively alleviates acute oxidative stress-induced growth retardation and intestinal injury via improving antioxidant capacity in piglets.Our findings may provide an efficient strategy for alleviating oxidative stress-induced inferior growth performance and intestinal damage in piglets.

关 键 词：SQUALENE Growth performance Oxidative stress Intestinal injury Antioxidant PIGLET 

分 类 号：S828.5[农业科学—畜牧学]