不同光照强度对一种新紫菜孢子体的光合色素和叶绿素荧光参数的影响  

Effects of Different Light Intensity on Photosynthetic Pigment and Chlorophyll Fluorescence Parameters of Neoporphyra sp.Sporophyte

作　　者：王楠 苏妮 顾全 李信书 刘建羽 WANG Nan;SU Ni;GU Quan(Key Laboratory of Marine Biotechnology,College of Marine Science and Fisheries,Jiangsu Ocean University,Lianyungang,Jiangsu 222005)

机构地区：[1]江苏海洋大学海洋科学与水产学院海洋生物技术重点实验室 [2]江苏省海洋生物产业技术协同创新中心 [3]江苏海洋大学海洋科学与水产学院

出　　处：《安徽农业科学》2024年第7期1-4,9,共5页Journal of Anhui Agricultural Sciences

基　　金：江苏省科技厅苏北科技专项(LYG-SZ201817);江苏省现代农业重点项目(BE2018335)。

摘　　要：将新紫菜孢子体放置在5种不同的光照强度下[10、20、40、80、160μmol/(m^(2)·s)]培养,测量叶绿素a(Chl a)、类胡萝卜素(Car)、藻红蛋白(PE)、最大光合效率(F_(v)/F_(m))和最大相对电子传递速率(rETR_(max)),探讨光照强度对新紫菜孢子体的光合色素含量和叶绿素荧光参数的影响。结果表明,20μmol/(m^(2)·s)培养下的新紫菜光合色素(Chl a、Car和PE)含量在培养期间都呈现出一个较高的数值,培养30 d后的新紫菜孢子体的F_(v)/F_(m)呈现出较高值。80和160μmol/(m^(2)·s)处理下培养至15 d时其叶绿素荧光参数(F_(v)/F_(m)和rETR_(max))呈现出较高的数值,培养至30 d时其光合色素含量和叶绿素荧光参数均呈现出一个低值。20μmol/(m^(2)·s)条件培养下的新紫菜孢子体色泽呈现健康的紫红色,高光[80、160μmol/(m^(2)·s)]培养下的新紫菜孢子体色泽变淡,发黄。根据其光合色素含量和叶绿素荧光参数来看,新紫菜孢子体最适培养光照强度应该在20μmol/(m^(2)·s)。The sporophytes of Neoporphyra sp.were placed in 5 different light intensities,respectively 10,20,40,80 and 160μmol/(m^(2)·s),respectively.Chlorophyll a(Chl a),carotenoids(Car),phycoerythrin(PE),maximum photosynthetic efficiency(F_v/F_m)and maximum relative electron transfer rate(rETR_(max))were measured,the effects of light intensity on photosynthetic pigment content and chlorophyll fluorescence parameters of Neoporphyra sp.sporophyte were explored.The result showed that the photosynthetic pigment contents(Chl a,Car,and PE)of N.sp.sporophyte by 20μmol/(m^(2)·s)were relatively high during the cultivation period,and the F_v/F_(m)of Neoporphyra sp.sporophyte after 30 days of cultivation showed a high value.The chlorophyll fluorescence parameters(F_v/F_(m)and rETR_(max))showed high values after cultured at 80 and 160μmol/(m^(2)·s)for 15 days,while the photosynthetic pigment content and chlorophyll fluorescence parameters showed low values after 30 days of culture.The sporophytes of Neoporphyra sp.were healthy purplish red in 20μmol/(m^(2)·s)cultures,while the sporophytes of Neoporphyra sp.were light and yellow in high light cultures[80 and 160μmol/(m^(2)·s)].According to the photosynthetic pigment content and chlorophyll fluorescence parameters,the optimal light intensity of Neoporphyra sp.spores was 20μmol/(m^(2)·s).

关 键 词：新紫菜 光照强度 光合色素 叶绿素荧光参数 

分 类 号：S968.31[农业科学—水产养殖]