外源贝莱斯芽孢杆菌对玉米种芽萌发·幼苗生长与光合系统的影响  

Effect of Exogenous Bacillus velezensis on Seed Germination and Seedling Growth and Photosynthetic System of Maize

作　　者：萧珣 王兴亚 张守梅 高琪 孙琳琳 陈利容[2] 马振萍 王红霞 龚魁杰 郭玉秋[2] 刘开昌[1,2] XIAO Xun;WANG Xing-ya;ZHANG Shou-mei(College of Agriculture,Qingdao Agricultural University,Qingdao,Shandong 266109;Crop Research Institute,Shandong Academy of Agricultural Sciences,Jinan,Shandong 250100)

机构地区：[1]青岛农业大学农学院,山东青岛266109 [2]山东省农业科学院作物研究所,山东济南250100 [3]滨州市农业科学院,山东滨州256600

出　　处：《安徽农业科学》2024年第7期36-40,共5页Journal of Anhui Agricultural Sciences

基　　金：国家重点研发计划项目(2022YFD2300805);山东省玉米产业技术体系项目(SDAIT-02-11);山东省技术创新引导计划项目(2018YFD0300605,2020LYXZ032,2021LYXZ004)。

摘　　要：为探究外源贝莱斯芽孢杆菌(B.v.)对玉米种芽萌发、幼苗生长及光合系统的调控作用,以郑单958和京农科728为供试材料接种B.v.。结果表明:与CK相比,外源施加B.v.能显著促进玉米种芽萌发,有效提升幼苗株高、叶面积、鲜重、干重与根冠比;与芽孢杆菌菌剂相比,B.v.处理在促进玉米种芽萌发方面具有显著优势,株高、叶面积、干重、鲜重等农艺性状有关指标也与芽孢杆菌菌剂无显著差异。与CK相比,外源B.v.处理下的玉米幼苗P_(n)、T_(r)、G_(s)、WUE显著提升,C_(i)较CK显著下降;B.v.处理下的P_(n)、T_(r)和G_(s)与芽孢杆菌菌剂处理无显著性差异,但在C_(i)利用效能方面显著弱于芽孢杆菌菌剂。与CK相比,外源施加B.v.有助于提升玉米幼苗叶片中叶绿素含量并改善荧光特性,显著提升叶绿素a、叶绿素b、叶绿素a/b、总叶绿素含量、F_(m)、F_(v)/F_(m)、F_(v)/F_(o)和q_(P),显著降低F_(o)和NPQ;B.v.处理下的叶片各叶绿素含量及总含量均显著高于芽孢杆菌菌剂处理,荧光参数整体与芽孢杆菌菌剂无显著差异,部分指标甚至优于芽孢杆菌菌剂处理。因此,贝莱斯芽孢杆菌在玉米上有着良好开发前景和推广应用价值,具有进一步开发成新型菌剂的潜力。To investigate the regulatory effects of exogenous Bacillus velezensis(B.v.)on seed germination,seedling growth and photosynthetic system of maize,Zhengdan 958 and Jingnongke 728 were used as test materials for inoculation with B.v..the results showed that:Compared with CK,exogenous application of B.v.significantly promoted maize seed shoot germination and effectively increased seedling height,leaf area,fresh weight,dry weight and root to crown ratio.Compared with Bacillus bacteria agent,B.v.treatment had a significant advantage in promoting maize seed germination,and the indexes related to agronomic traits such as plant height,leaf area,dry weight and fresh weight were also not significantly different from those of Bacillus bacteria agent.Compared to CK,maize seedlings under exogenous B.v.treatment showed significantly higher P_(n),T_(r),G_(s)and WUE and significantly lower C_(i)compared to CK.P_(n),T_(r)and G_(s)under B.v.treatment were not significantly different from Bacillus bacteria agent treatment,but significantly weaker than Bacillus mycoflora in terms of C_(i)utilization efficiency.Compared with CK,exogenous application of B.v.helped to enhance chlorophyll content and improve fluorescence characteristics in maize seedling leaves:significantly enhanced chlorophyll a,chlorophyll b,chlorophyll a/b,the total chlorophyll content,F_m,F_v/F_m,F_v/F_(oand)q_P,and significantly reduced F_(o)and NPQ.The chlorophyll content and total content of leaves under B.v.treatment were significantly higher than those under Bacillus bacteria agent treatment,and the fluorescence parameters were not significantly different from Bacillus bacteria agent treatment overall,and some indexes were even better than Bacillus bacteria agent treatment.Therefore,Bacillus velezensis has a good development prospect and application value on corn,and has the potential to be further developed into a new type of bacterial agent.

关 键 词：贝莱斯芽孢杆菌 种芽萌发 光合特性 叶绿素含量 叶绿素荧光参数 

分 类 号：S513[农业科学—作物学]