丹参LBD基因家族的特性鉴定与抗热性分析  

Genome-wide Identification and Analysis of LBD Gene Family in Salvia miltiorrhiza

作　　者：李万 樊昕华 LI Wan;FAN Xin-hua(School of Biopharmaceutical and Food Engineering,Shangluo University,Shangluo 726000,Shaanxi)

机构地区：[1]商洛学院生物医药与食品工程学院,陕西商洛726000

出　　处：《商洛学院学报》2024年第2期66-74,共9页Journal of Shangluo University

基　　金：陕西省教育厅科学研究计划项目(23JK0415)。

摘　　要：利用生物信息学方法,对丹参LBD(later organ boundaries domain)家族成员(SmLBDs)进行了系统的筛选和鉴定,并分析了其在高温胁迫(37℃)下的表达模式。结果表明,丹参中共有52个SmLBDs,可分为groupⅠ、groupⅡ、groupⅢ、groupⅣ、groupⅤ、groupⅥ和groupⅦ共7组。52个SmLBDs编码蛋白的氨基酸残基数为103~298,相对分子质量为11.29~31.72 k Da,等电点分布于4.44~10.71,均为亲水性蛋白,且大多定位在细胞核中。此外,分析52个SmLBDs在高温胁迫(37℃)下的表达模式发现,7个基因的表达水平下降,37个基因的表达水平上升,尤其是SmLBD8、SmLBD40和SmLBD44的表达水平显著提高,表明这些基因可能与丹参的抗热性相关。The members of the later organ boundaries domain(LBD)family of Salvia miltiorrhia LBD(SmLBDs)were systematically screened and identified using bioinformatics methods,and their expression patterns were analyzed under high temperature stress(37℃).The results showed that there were 52 SmLBDs in Salvia miltiorrhiza,which could be divided into 7 groups:groupⅠ,groupⅡ,groupⅢ,groupⅣ,groupⅤ,groupⅥand groupⅦ.The amino acid residues of the 52 proteins encoded by SmLBDs ranged from 103 to 298,the relative molecular weights ranged from 11.29 to 31.72 kDa,and the isoelectric points were distributed from 4.44 to 10.71.All of 52 SmLBDs were hydrophilic proteins,and most of them were located in the nucleus.In addition,the expression patterns of 52 SmLBDs under high temperature stress(37℃)were analyzed,and the expression levels of 7 genes were decreased,and 37 genes were increased,especially the expression level of SmLBD8,SmLBD40 and SmLBD44was significantly increased,indicating that these genes may be related to the heat resistance of Salvia miltiorrhiza.

关 键 词：丹参 LBD家族 亚细胞定位 高温胁迫 

分 类 号：S567.23[农业科学—中草药栽培]