Histone H3 lysine 27 trimethylation suppresses jasmonate biosynthesis and signaling to affect male fertility under high temperature in cotton  

作　　者：Yanlong Li Miao Chen Aamir Hamid Khan Yizan Ma Xin He Jing Yang Rui Zhang Huanhuan Ma Chunyang Zuo Yawei Li Jie Kong Maojun Wang Longfu Zhu Xianlong Zhang Ling Min 

机构地区：[1]National Key Laboratory of Crop Genetic Improvement&Hubei Hongshan Laboratory,Huazhong Agricultural University,Wuhan 430070,Hubei,China [2]Institute of Economic Crops,Xinjiang Academy of Agricultural Sciences,Urumqi 830091,Xinjiang,China

出　　处：《Plant Communications》2023年第6期96-112,共17页植物通讯（英文）

基　　金：supported by the Major Project of Hubei Hongshan Laboratory(2022hszd004);the National Cotton Production System,Ministry of Agriculture and Rural Affairs of China(CARS-15-04);the National Key R&D Program of China(2022YFF1003502);the National Natural Science Foundation of China(32072024).

摘　　要：High-temperature(HT)stress causes male sterility in crops,thus decreasing yields.To explore the possible contribution of histone modifications to male fertility under HT conditions,we defined the histone methylation landscape for the marks histone H3 lysine 27 trimethylation(H3K27me3)and histone H3 lysine 4 trimethylation(H3K4me3)by chromatin immunoprecipitation sequencing(ChIP-seq)in two differing upland cotton(Gossypium hirsutum)varieties.We observed a global disruption in H3K4me3 and H3K27me3 modifications,especially H3K27me3,in cotton anthers subjected to HT.HT affected the bivalent H3K4me3–H3K27me3 modification more than either monovalent modification.We determined that removal of H3K27me3 at the promoters of jasmonate-related genes increased their expression,maintaining male fertility under HT in the HT-tolerant variety at the anther dehiscence stage.Modulating jasmonate homeostasis or signaling resulted in an anther indehiscence phenotype under HT.Chemical suppression of H3K27me3 deposition increased jasmonic acid contents and maintained male fertility under HT.In summary,our study provides new insights into the regulation of male fertility by histone modifications under HT and suggests a potential strategy for improving cotton HT tolerance.

关 键 词：high temperature H3K27me3 H3K4me3 cotton anther JASMONATE 

分 类 号：Q75[生物学—分子生物学]