VAMP726 from maize and Arabidopsis confers pollen resistance to heat and UV radiation by influencing lignin content of sporopollenin  

作　　者：Wenqi Yang Dongdong Yao Haiyang Duan Junli Zhang Yaling Cai Chen Lan Bing Zhao Yong Mei Yan Zheng Erbing Yang Xiaoduo Lu Xuehai Zhang Jihua Tang Ke Yu Xuebin Zhang 

机构地区：[1]State Key Laboratory of Crop Stress Adaptation and Improvement,Henan Joint International Laboratory for Crop Multi-Omics Research,School of Life Sciences,Henan University,Kaifeng 475004,China [2]National Key Laboratory of Wheat and Maize Crop Science,College of Agronomy,Henan Agricultural University,Zhengzhou 450002,China [3]The Shennong Laboratory,Zhengzhou 450002,China [4]College of Chemistry,Zhengzhou University,Zhengzhou 450001,China [5]National Engineering Laboratory of Crop Stress Resistance,School of Life Science,Anhui Agricultural University,Hefei 230036,China

出　　处：《Plant Communications》2023年第6期163-178,共16页植物通讯（英文）

基　　金：supported by grants from the National Natural Science Foundation of China(31970323,32170269 to Xuebin Zhang,32171980 to Xuehai Zhang);the Henan Key Scientific Research Programs to Universities and Colleges(22ZX006 to Xuebin Zhang);the Open Project Funding of the State Key Laboratory of Crop Stress Adaptation and Improvement(2021KF07 to Xuehai Zhang);the Open Project Funding of the State Key Laboratory of Wheat and Maize Crops Science(30501194 to K.Y.);the Henan Overseas Expertise Introduction Center for Discipline Innovation(CXJD2020004);the Program for Innovative Research Team(in Science and Technology)of the University of Henan Province(21IRTSTHN019).

摘　　要：Sporopollenin in the pollen cell wall protects male gametophytes from stresses.Phenylpropanoid derivatives,including guaiacyl(G)lignin units,are known to be structural components of sporopollenin,but the exact composition of sporopollenin remains to be fully resolved.We analyzed the phenylpropanoid derivatives in sporopollenin from maize and Arabidopsis by thioacidolysis coupled with nuclear magnetic resonance(NMR)and gas chromatography–mass spectrometry(GC–MS).The NMR and GC–MS results confirmed the presence of p-hydroxyphenyl(H),G,and syringyl(S)lignin units in sporopollenin from maize and Arabidopsis.Strikingly,H units account for the majority of lignin monomers in sporopollenin from these species.We next performed a genome-wide association study to explore the genetic basis of maize sporopollenin composition and identified a vesicle-associated membrane protein(ZmVAMP726)that is strongly associated with lignin monomer composition of maize sporopollenin.Genetic manipulation of VAMP726 affected not only lignin monomer composition in sporopollenin but also pollen resistance to heat and UV radiation in maize and Arabidopsis,indicating that VAMP726 is functionally conserved in monocot and dicot plants.Our work provides new insight into the lignin monomers that serve as structural components of sporopollenin and characterizes VAMP726,which affects sporopollenin composition and stress resistance in pollen.

关 键 词：pollen cell wall SPOROPOLLENIN lignin monomers heat stress UV radiation 

分 类 号：Q94[生物学—植物学]