Adenine base editor incorporating the N-methylpurine DNA glycosylase MPGv3 enables efficient A-to-K base editing in rice  

作　　者：Xuemei Wu Bin Ren Lang Liu Shengqun Qiu Xin’ge Li Peijing Li Fang Yan Honghui Lin Xueping Zhou Dawei Zhang Huanbin Zhou 

机构地区：[1]Ministry of Education Key Laboratory for Bio-Resource and Eco-Environment,College of Life Science,State Key Laboratory of Hydraulics and Mountain River Engineering,Sichuan University,Chengdu 610064,China [2]State Key Laboratory for Biology of Plant Diseases and Insect Pests,Institute of Plant Protection,Chinese Academy of Agricultural Sciences,Beijing 100193,China [3]Scientific Observing and Experimental Station of Crop Pests in Guilin,Ministry of Agriculture and Rural Affairs,Guilin 541399,China [4]Department of Plant Pathology,China Agricultural University,Beijing 100193,China [5]State Key Laboratory of North China Crop Improvement and Regulation,College of Plant Protection,Hebei Agricultural University,Baoding 071000,China [6]School of Life Science and Technology,Hubei Engineering University,Xiaogan 432000,China [7]State Key Laboratory of Rice Biology,Institute of Biotechnology,Zhejiang University,Hangzhou 310058,China [8]Hainan Yazhou Bay Seed Lab/National Nanfan Research Institute(Sanya),Chinese Academy of Agricultural Sciences,Sanya 572024,China

出　　处：《Plant Communications》2023年第6期230-233,共4页植物通讯（英文）

基　　金：supported by the Hainan Yazhou Bay Seed Lab(B21HJ0215);the Key Laboratory of Gene Editing Technologies(Hainan)of the Ministry of Agriculture and Rural Affairs,China,and the Agricultural Science and Technology Innovation Program of the Chinese Academy of Agricultural Sciences to H.Z.and the Central Public-interest Scientific Institution Basal Research Fund(Y2022PT24)to F.Y.

摘　　要：Dear Editor,Crop genetic diversity and elite agronomic traits are mainly caused by genetic variants,approximately half of which are single-nucleotide polymorphisms.Precise nucleotide substitution through CRISPR–Cas-mediated base editors has been widely used to correct defective alleles and create novel alleles by artificial evolution for rapid crop genetic improvement.Since 2017,cytosine base editors(CBEs)and adenine base editors(ABEs)have been successively developed in many plant species and have been continuously optimized to generate highly efficient C-to-T and A-to-G transitions,as well as by-product C-to-A/G conversion with low efficiency(Ren et al.,2018;Yan et al.,2021).

关 键 词：ELITE BASE EDITOR 

分 类 号：S511[农业科学—作物学]