复合型抗盐剂对NaCl胁迫下小麦种子萌发和生长的影响  

Effects of compound anti-salt agent on seed germination and growth of wheat under NaCl stress

作　　者：刘晓晨 王树凤[1] 寿松涛 张建锋[1] LIU Xiaochen;WANG Shufeng;SHOU Songtao;ZHANG Jianfeng(Research Institute of Subtropical Forestry,Chinese Academy of Forestry,Hangzhou,Zhejiang 311400,China;Nanjing Forestry University,Nanjing,Jiangsu 210037,China;Center for Rural Vitalization of Fuyang,Hangzhou,Zhejiang 311400,China)

机构地区：[1]中国林业科学研究院亚热带林业研究所,浙江杭州311400 [2]南京林业大学,江苏南京210037 [3]杭州市富阳区乡村振兴服务中心,浙江杭州311400

出　　处：《干旱地区农业研究》2024年第2期199-209,共11页Agricultural Research in the Arid Areas

基　　金：浙江省重点研发计划项目(2021C02038)。

摘　　要：将KNO_(3)、CaCl_(2)、DA-6、KH_(2) PO_(4)、FeSO_(4)·7H_(2) O、MgSO_(4)·7H_(2) O和蔗糖7种物质组合配制复合型抗盐剂,以‘济麦22’为研究材料,采用水培法,探讨了不同浓度(0.2%、0.4%和0.6%)盐(NaCl)胁迫下,施加不同浓度(10%、20%和30%)抗盐剂对种子萌发及后续幼苗生长的影响,并提出了适合小麦的抗盐剂配方。结果表明:(1)30%、20%、10%抗盐剂分别对0.2%、0.4%、0.6%NaCl胁迫下小麦种子萌发的促进效果最好,发芽率分别提高了6.4%、12.0%、26.5%。(2)0.6%NaCl胁迫下添加抗盐剂,根干质量显著增加了29.7%~35.9%;在抗盐剂作用下,光合速率分别提高了67.5%~90.0%(0.2%NaCl胁迫)、13.3%~51.5%(0.4%NaCl胁迫)和0.4%~25.5%(0.6%NaCl胁迫),且30%抗盐剂显著增强了0.2%NaCl胁迫下小麦的光合作用,增幅达90.0%。添加抗盐剂使小麦的光合色素含量升高,其中叶绿素a在0.2%和0.4%NaCl胁迫下分别升高了22.5%~39.0%和41.7%~43.2%,叶绿素b分别升高了22.1%~31.0%和14.7%~22.5%,类胡萝卜素分别升高了12.2%~28.6%和24.4%~32.3%。此外,其他各项生理指标也有相应改善。(3)主成分分析结果显示,盐胁迫下30%抗盐剂处理后小麦的得分均为最高,为最适浓度配比。Seven substances,including KNO_(3)、CaCl_(2)、DA-6、KH_(2) PO_(4)、FeSO_(4)·7H_(2) O、MgSO_(4)·7H_(2) O,and sucrose were used to formulate compound anti-salt agent.Using‘Jimai 22’as test material,the water cultivaton method was employed to investigate the effects of different concentrations of anti-salt agent(10%,20%and 30%)on seed germination and subsequent seedling growth under different salt(NaCl)stress(0.2%,0.4%and 0.6%),and consequently the suitable formulation of anti-salt agent for wheat was proposed.The results showed that:(1)30%,20%and 10%anti-salt agent had the best effect in promoting wheat seed germination under 0.2%,0.4%and 0.6%salt stress,and the germination rate increased by 6.4%,12.0%and 26.5%,respectively.(2)Adding anti-salt agents at a salt concentration of 0.6%,root dry mass significantly increased by 29.7%to 35.9%.Photosynthetic rate was increased after the addition of anti-salt agent by 67.5%~90.0%(salt concentration 0.2%),13.3%~51.5%(salt concentration 0.4%)and 0.4%~25.5%(salt concentration 0.6%),respectively.The 30%anti-salt agent significantly enhanced photosynthesis at a salt concentration of 0.2%,with an increase of 90.0%.The photosynthetic pigment content of wheat was increased,with Chla content increased by 22.5%~39.0%(salt concentration 0.2%),41.7%to 43.2%(salt concentration 0.4%);and Chlb content increased by 22.1%~31.0%(salt concentration 0.2%),14.7%~22.5%(salt concentration 0.4%);and carotenoid content increased by 12.2%to 28.6%(salt concentration 0.2%)and 24.4%to 32.3%(salt concentration 0.4%),respectively.In addition,other physiological indicators also had corresponding improvement.(3)Based on the principal component analysis,the results showed that wheat treated with 30%anti-salt agent had the highest score,so 30%anti-salt agent was the most suitable concentration ratio.

关 键 词：复合抗盐剂 小麦 盐胁迫 种子萌发 抗性生理 

分 类 号：S512.1[农业科学—作物学] S473[生物学—植物学] Q945.78