CD226在小鼠小肠3型固有淋巴样细胞(ILC3)中的表达  

Expression of CD226 in the small intestinal group 3 innate lymphoid cells(ILC3)in mice

作　　者：羊璐 马菁昌 刘懿天 王婷婷 王玉玲 庄然 张正祥[1] YANG Lu;MA Jingchang;LIU Yitian;WANG Tingting;WANG Yuling;ZHUANG Ran;ZHANG Zhengxiang(Medical College of Yan’an University,Yan’an 716000;Department of Immunology,Basic Medical Science Academy,Air Force Medical University,Xi’an 710032,China)

机构地区：[1]延安大学医学院,陕西延安716000 [2]空军军医大学基础医学院免疫学教研室,陕西西安710032

出　　处：《细胞与分子免疫学杂志》2024年第1期1-6,共6页Chinese Journal of Cellular and Molecular Immunology

基　　金：国家自然科学基金(81871258);延安大学科研计划项目(YDY2020-21)。

摘　　要：目的 观察CD226在小肠3型固有淋巴样细胞(ILC3)上的表达情况。方法 采用生物信息学方法分析小鼠CD226在ILC中的表达。分离正常C57BL/6J小鼠小肠黏膜固有层淋巴细胞(LPL),流式细胞术检测CD226在ILC1和ILC3上的表达。构建葡聚糖硫酸钠(DSS)诱导小鼠肠炎模型,观察CD226在ILC3上表达的变化情况。结果 小鼠小肠ILC1和ILC3均表达CD226;肠炎模型中ILC3所占比例降低,但CD226在ILC3上的表达水平升高。结论 小鼠小肠表达CD226,DSS诱导的肠炎模型中虽然ILC3比例降低,但CD226在ILC3的表达增加。Objective To observe the expression of adhesion molecule CD226 on the small intestinal group 3 innate lymphoid cells(ILC3)in mice.Methods The bioinformatics was used to analyze the expression of CD226 on murine ILCs.Small intestinal mucosal lamina propria lymphocytes(LPL)were isolated from wild-type C57BL/6J mice,and the expression of CD226 on ILC1 and ILC3 was detected by flow cytometry.A mouse model of dextran sulfate sodium(DSS)-induced colitis was constructed to observe the changes in the expression of CD226 on ILC3.Results Both ILC1 and ILC3 in the mice small intestine expressed CD226 molecules;the proportion of ILC3 was reduced,while the expression level of CD226 on ILC3 was increased in the colitis model.Conclusion CD226 is expressed on the small intestines of mice,and although the proportion of ILC3 decreases in the DSS-induced colitis,the expression of CD226 on ILC3 increases.

关 键 词：3型固有淋巴样细胞(ILC3) CD226 黏膜免疫 肠道 

分 类 号：R392.9[医药卫生—免疫学] R574[医药卫生—基础医学] R574.5