ITP患者PD-1/PD-L1表达特点及其在Treg与Breg细胞之间的相互作用机制分析  

Expression characteristics of PD-1/PD-L1 in ITP patients and analysis of interaction mechanism between Treg and Breg cells

作　　者：许腾[1] 崔彦杰[1] 李智伟[1] 刘红春[1] 郝立君[1] XU Teng;CUI Yanjie;LI Zhiwei;LIU Hongchun;HAO Lijun(Clinical Laboratory Center,The People′s Hospital of Xinjiang Uygur Autonomous Region,Urumqi 830001,China)

机构地区：[1]新疆维吾尔自治区人民医院临床检验中心,新疆乌鲁木齐830001

出　　处：《西部医学》2024年第4期608-613,共6页Medical Journal of West China

基　　金：新疆维吾尔自治区自然科学基金项目(2021D01C152)。

摘　　要：目的探讨原发免疫性血小板减少症(ITP)患者细胞程序性死亡受体-1(PD-1)/细胞程序性死亡配体1(PD-L1)表达特点及其在调节性T细胞(Treg)、调节性B细胞(Breg)间的相互作用。方法选取2018年12月—2022年1月在我院治疗的ITP患者106例作为观察组,其中轻度患者32例,中度患者44例,重度患者30例。同时选取同期健康志愿者100例作为对照组。检测两组Treg细胞百分比、Breg细胞百分比、Treg细胞表面PD-1阳性率、Breg细胞表面PD-L1阳性率等,同时分析观察组不同病情程度患者各指标差异。结果观察组Breg细胞百分比、Treg细胞百分比、TGF-β、IL-10和IL-4水平均明显低于对照组(P<0.05);观察组Treg细胞表面PD-1阳性率、Breg细胞表面PD-L1阳性率、可溶性程序性细胞死亡蛋白-1(sPD-1)和IL-17水平均明显高于对照组(均P<0.05);两组可溶性程序性细胞死亡蛋白配体-1(sPD-L1)水平比较差异无统计学意义(P>0.05)。观察组重度患者Breg细胞百分比、Treg细胞百分比均明显低于轻度和中度患者(均P<0.05),而Treg细胞表面PD-1阳性率、Breg细胞表面PD-L1阳性率均明显高于轻度和中度患者(均P<0.05)。Treg细胞表面PD-1阳性率与Breg细胞表面PD-L1阳性率呈正相关(r=0.446,P<0.05)。观察组治疗后Breg细胞百分比、Treg细胞百分比、TGF-β、IL-10和IL-4水平有所升高(P<0.05),而Treg细胞表面PD-1阳性率、Breg细胞表面PD-L1阳性率、sPD-1和IL-17水平有所降低(P<0.05),治疗前后sPD-L1水平比较差异无统计学意义(P>0.05)。结论ITP患者Treg细胞表面PD-1和Breg细胞表面PD-L1阳性率明显升高,与患者病情严重程度呈正相关,同时Treg细胞表面PD-1和Breg细胞表面PD-L1表达之间存在相关性。Objective To investigate the expression of programmed cell death receptor-1(PD-1)/programmed cell death ligand 1(PD-L1)in patients with primary immune thrombocytopenia(ITP)and the interaction between regulatory T cells(Treg)and regulatory B cells(Breg).Methods 106 ITP patients treated in our hospital from December 2018 to January 2022 were selected as the observation group,including 32 mild patients,44 moderate patients and 30 severe patients.At the same time,100 healthy volunteers were selected as the control group,and the Treg cells,Breg cells,the positive rate of PD-1 on the surface of Treg cells,and the positive rate of PD-L1 on the surface of Breg cells and so on were detected in the two groups.At the same time,the differences of various indicators in patients with different disease severity in the observation group were analyzed.Results Breg cells,Treg cells,transforming growth factor-β(TGF-β),interleukin-10(IL-10)and interleukin-4(IL-4)in the observation group were(4.51±1.02)%,(5.02±1.00)%,(2156.65±354.43)pg/ml,(4.10±0.99)pg/ml and(301.12±65.56)pg/ml,respectively,which were significantly lower than those in the control group(P<0.05).The positive rates of PD-1 on the surface of Treg cells,PD-L1 on the surface of Breg cells,soluble programmed cell death protein-1(sPD-1)and interleukin-17(IL-17)in the observation group were(28.34±9.22)%,(12.19±2.11)%,(102.87±15.56)pg/ml and(11.45±2.32)pg/ml,respectively,which were significantly higher than those in the control group(P<0.05).There was no significant difference in soluble programmed cell death protein ligand 1(sPD-L1)between the observation group and the control group(P>0.05).The Breg cells and Treg cells of the severe patients in the observation group were(3.52±0.94)%and(3.80±1.02)%,respectively,which were significantly lower than those of the mild and moderate patients(P<0.05).The positive rates of PD-1 on Treg cells and PD-L1 on Breg cells were(32.53±7.04)%and(14.14±2.01)%,respectively,which were significantly higher than those of mild and mod

关 键 词：原发免疫性血小板减少症 细胞程序性死亡受体-1 细胞程序性死亡配体1 调节性T细胞 调节性B细胞 

分 类 号：R558.2[医药卫生—血液循环系统疾病]