胃癌组织中KAI1/CD82、p27和p53的表达水平及其临床意义  

Expression of KAI1/CD82,p27,and p53 in gastric cancer tissue and their clinical significance

作　　者：崔荣花[1] 李建旺[1] 元建华[1] 张曙波 毛山山[1] 陈琼慧 李兴[1] 张玮芳 谢宗宙[1] CUI Rong-hua;LI Jian-wang;YUAN Jian-hua;ZHANG Shu-bo;MAO Shan-shan;CHEN Qiong-hui;LI Xing;ZHANG Wei-fang;XIE Zong-zhou(Department of Oncology,the Affiliated Haikou Hospital of Xiangya Medical College,Central South University,Haikou 570208,Hainan,CHINA)

机构地区：[1]中南大学湘雅医学院附属海口医院肿瘤科,海南海口570208

出　　处：《海南医学》2024年第7期978-981,共4页Hainan Medical Journal

基　　金：海南省卫生健康行业科研项目(编号:21A200338、22A200306)。

摘　　要：目的研究胃癌组织中KAI1/CD82、p27、p53蛋白的表达水平及其临床意义。方法收集中南大学湘雅医学院附属海口医院胃肠外科2020年6月至2021年6月期间接受胃癌切除术治疗的43例胃癌患者标本,将标本分为43例胃癌组、43例配对的癌旁组(距癌2 cm)和43例配对的正常组(距癌5 cm以上),运用免疫印迹(Western blot)方法检测并比较三组组织中的KAI1/CD82、p27、p53蛋白表达水平,分析胃癌组织中KAI1/CD82、p27、p53的表达水平与临床病理参数的关系,采用Pearson相关分析法分析其与胃癌的临床病理特征的关系。结果胃癌组中的KAI1/CD82、p27、p53蛋白表达水平明显低于癌旁组和正常组,且癌旁组明显低于正常组,差异均有统计学意义(P<0.05);KAI1/CD82、p27、p53蛋白的阳性表达率与肿瘤组织的分化程度、临床分期和淋巴结是否转移有关(P<0.05),而与年龄、性别无关(P>0.05);经Pearson相关分析结果显示,胃癌组织中KAI1/CD82与p27蛋白和p53蛋白的表达均呈正相关(r=0.586、0.426,P<0.05)。结论胃癌组织中的KAI1/CD82、p27、p53蛋白表达水平明显降低,联合检测KAI1/CD82、p27、p53可能成为胃癌诊断及预后评估的新的分子标记物。Objective To investigate expression levels of KAI1/CD82,p27,and p53 protein in gastric cancer tissues and assess their clinical significance.Methods Specimens were collected from 43 gastric cancer patients who underwent gastrectomy at the Department of Gastrointestinal Surgery,the Affiliated Haikou Hospital of Xiangya Medical College,Central South University,from June 2020 to June 2021.The specimens were divided into three groups:43 cases of gastric cancer group(gastric cancer tissue),43 cases of pericancerous group(paired pericancerous tissue,2 cm from the cancer),and 43 cases of normal group(paired normal tissue,more than 5 cm from the cancer).The expression levels of KAI1/CD82,p27,and p53 proteins in the three groups of tissues were detected and compared using the Western blot.And the relationship between the expression levels of KAI1/CD82,p27,and p53 in gastric cancer tissue and clinical pathological parameters was analyzed.The relationship between their expressions and the clinical pathological characteristics of gastric cancer was assessed using Pearson correlation analysis.Results Expression levels of KAI1/CD82,p27,and p53 proteins were significantly lower in gastric cancer group compared to pericancerous group and normal group.Positive expression of KAI1/CD82,p27,and p53 was associated with tumor differentiation,clinical staging,and lymph node metastasis(P<0.05),but not with age or gender(P>0.05).Pearson correlation analysis showed positive correlations among KAI1/CD82,p27,and p53 protein expressions in gastric cancer tissues(r=0.586,0.426,P<0.05).Conclusion KAI1/CD82,p27,and p53 protein expressions are significantly reduced in gastric cancer tissues.Combined detection of these proteins could serve as novel molecular markers for diagnosing and prognosticating gastric cancer.

关 键 词：胃癌 KAI1/CD82 P27 P53 免疫印迹 诊断 预后 

分 类 号：R735.2[医药卫生—肿瘤]