基于抗炎作用的蒲公英清热解毒物质基础研究  

Study on Pugongying(Taraxaci Herba)Material Basis for Clearing Heat and Detoxifying Based on Anti-inflammatory Effect

作　　者：孙洋洋 赵宁[1,2] 柏冬[1] SUN Yangyang;ZHAO Ning;BAI Dong(Institute of Basic Theory of Traditional Chinese Medicine,China Academy of Chinese Medical Sciences,Beijing 100007,China;Xiyuan Hospital,China Academy of Chinese Medical Sciences,Beijing 100091,China)

机构地区：[1]中国中医科学院中医基础理论研究所,北京100007 [2]中国中医科学院西苑医院,北京100091

出　　处：《中华中医药学刊》2024年第4期92-96,共5页Chinese Archives of Traditional Chinese Medicine

基　　金：国家自然科学基金项目(81403282);中国癌症基金会建生鲜药创研基金项目(JSJC-20190102-044);中国中医科学院自主选题研究项目(YZ202109)。

摘　　要：目的探究蒲公英清热解毒的药效物质基础。方法采用二甲苯致小鼠耳肿胀模型,比较蒲公英鲜品匀浆、鲜品水提物和蒲公英干品水提物的抗炎作用差异;通过高效液相色谱-串联质谱法(HPLC-MS/MS),对比蒲公英鲜品匀浆、鲜品水提物和蒲公英干品水提物中次生物质木犀草苷、绿原酸、阿魏酸、咖啡酸、秦皮乙素5种成分含量的差异;采用苯酚-硫酸比色法和考马斯亮蓝法分别对比蒲公英鲜品匀浆、鲜品水提物和蒲公英干品水提物中初生物质多糖和蛋白含量的差异;通过离子交换色谱法分离纯化蒲公英鲜品中的多糖和蛋白类成分,进一步研究其清热解毒的物质基础。结果蒲公英鲜品匀浆、鲜品水提物和蒲公英干品水提物组小鼠的耳肿胀率分别为0.59、0.85、0.94,其中蒲公英鲜品匀浆提取物中次生物质的含量最低,而初生物质多糖和蛋白类成分含量最高,多糖组和蛋白组小鼠的耳肿胀率分别为0.45、0.74。结论蒲公英鲜品的抗炎作用优于干品,而且鲜品和干品中次生物质含量差异较小,初生物质中多糖和蛋白的含量差异大,通过进一步研究分离纯化后多糖和蛋白的抗炎作用差异,结果表明蒲公英鲜品中的多糖类成分是发挥清热解毒作用的主要物质基础。Objective To explore the pharmacodynamic basis of Pugongying(Taraxaci Herba)for clearing heat and detoxifying.Methods The anti-inflammatory effects of Pugongying(Taraxaci Herba)fresh homogenate,fresh water extract and dried Pugongying(Taraxaci Herba)water extract were compared by using xylene-induced ear swelling model.High performance liquid chromatography-tandem mass spectrometry(HPLC-MS/MS)method was used to compare the contents of five components including sub-biomass luteolin,chlorogenic acid,ferulic acid,caffeic acid and ethyl pigment in Pugongying(Taraxaci Herba)fresh homogenate,fresh water extract and dried Pugongying(Taraxaci Herba)water extract.The phenol-sulfuric acid colorimetric method and Coomassie brilliant blue method were used to compare the differences of primary biomass polysaccharides and protein contents in Pugongying(Taraxaci Herba)fresh homogenate,fresh water extract and dried Pugongying(Taraxaci Herba)water extract,respectively.The polysaccharides and protein components in Pugongying(Taraxaci Herba)fresh products were separated and purified by ion exchange chromatography,and the material basis for clearing heat and detoxification was further studied.Results The ear swelling rates of Pugongying(Taraxaci Herba)fresh homogenate,fresh water extract and dried Pugongying(Taraxaci Herba)water extract group were 0.59,0.85 and 0.94,respectively,among which the content of sub-biomass in Pugongying(Taraxaci Herba)fresh homogenate extract was the lowest,while the contents of primary biomass polysaccharides and protein components were the highest,and the ear swelling rates of mice in the polysaccharide group and protein group were 0.45 and 0.74,respectively.Conclusion The anti-inflammatory effect of Pugongying(Taraxaci Herba)fresh products is better than that of dried products,and the difference between the content of sub-biomass in fresh and dried products is small,and the contents of polysaccharides and proteins in primary biomass are very different.

关 键 词：蒲公英鲜品 成分差异 抗炎 多糖类成分 物质基础 

分 类 号：R282.710.5[医药卫生—中药学]