五指毛桃饮片的质量标准研究  

Study on Quality Standard of Wuzhimaotao(Fici Hirtae Radix)Decoction Pieces

作　　者：梁永娟 韦礼校 苏静红 李锦清 陈清[2] 林青华 蒋林 LIANG Yongjuan;WEI Lixiao;SU Jinghong;LI Jinqing;CHEN Qing;LIN Qinghua;JIANG Lin(College of Pharmacy,Guangxi University of Chinese Medicine,Nanning Guangxi 530200,China;Guangxi Zhuang Yao Medicine Center of Engineering and Technology,Guangxi University of Chinese Medicine,Nanning Guangxi 530200,China)

机构地区：[1]广西中医药大学药学院,广西南宁530200 [2]广西中医药大学广西壮瑶药工程技术研究中心,广西南宁530200

出　　处：《中医药导报》2024年第3期80-86,共7页Guiding Journal of Traditional Chinese Medicine and Pharmacy

基　　金：广西重点研发计划项目(桂科AB21196051)。

摘　　要：目的:完善五指毛桃饮片的质量标准。方法:收集不同产地的五指毛桃饮片29批,对其进行外观性状、粉末鉴别和薄层鉴别,并测定水分、总灰分、酸不溶性灰分、水溶性浸出物的含量;采用HPLC法测定其补骨脂素、佛手柑内酯的含量;建立其HPLC特征图谱,以补骨脂素(4号峰)为参照,采用《中药色谱指纹图谱相似度评价系统(2012年版)》进行相似度评价,并确定共有峰。结果:五指毛桃饮片呈类圆形或不规则的厚片,表皮灰棕色至红褐色,皮部与木部易分离,质硬而脆,断面纤维性;粉末灰黄白色,可见石细胞、草酸钙方晶、淀粉粒、木栓细胞等;薄层鉴别结果发现,供试品和对照品在薄层板相应的位置显相同颜色的斑点;其水分、总灰分、酸不溶性灰分、水溶性浸出物、补骨脂素和佛手柑内酯的含量分别为5.67%~11.29%、1.90%~3.85%、0.06%~0.69%、5.71%~19.17%、0.01%~0.36%和0.01%~0.18%,平均值分别为8.02%、2.47%、0.23%、12.16%、0.17%和0.05%,拟定其水分、总灰分和酸不溶性灰分的含量分别不得超过12.0%、4.0%、0.7%,水溶性浸出物、补骨脂素和佛手柑内酯的含量分别不得低于6.0%、0.02%和0.01%;其特征图谱的相似度大于0.900,共确认了11个共有峰,指认4、5号峰分别为补骨脂素、佛手柑内酯。结论:所建标准可为科学评价五指毛桃饮片的质量提供参考。Objective:To improve the quality standard of Wuzhimaotao(Fici hirtae radix)decoction pieces(FRDP).Methods:Twenty-nine batches of FRDP were collected from different producing areas to perform appearance and property identification,microscopic identification and thin layer chromatography(TLC)identification.Moisture,total ash,acid-insoluble ash and water-soluble extract were detected,and the content of psoralen and bergapten were determined by HPLC.HPLC method was used to establish the characteristic chromatogram of 29 batches of FRDP.Using Psoralen(NO.4 peak)as reference,similarity evaluation was conducted by using Similarity Evaluation System for TCM Chromatographic Fingerprints(2012 edition),and the common peaks were determined.Results:FRDP was like round or irregular thick pieces,and the surface was gray brown to reddish-brown.Its phloem was easily shed from xylem.It was hard and brittle,cross-section fiber.Stone cells,calcium oxalate square crystals,starch granules,cork cells and so on could be seen in the power of FRDP.The results of TLC identification showed that there were spots of the same color in the chromatographic position corresponding to the chromatographic position of psoralen.The average contents of water,total ash,acid-insoluble ash,water soluble extract,psoralen and bergapten were 5.67%-11.29%,1.90%-3.85%,0.06%-0.69%,5.71%-19.17%,0.01%-0.36%,0.01%-0.18%,respectively.The average values were 8.02%,2.47%,0.23%,12.16%,

关 键 词：五指毛桃饮片 质量标准 特征图谱 含量测定 补骨脂素 佛手柑内酯 

分 类 号：R284.1[医药卫生—中药学]