心可舒片改善射血分数保留心力衰竭大鼠心脏功能及其作用机制探讨  

Effects of Xinkeshu Tablet on Cardiac Function in Rats with Heart Failure with Preserved Ejection Fraction and Its Mechanism

作　　者：杨晨光 石玉姣 乔文博 刘永成 刘思雨 李知轩 董国菊[1,3] YANG Chen-guang;SHI Yu-jiao;QIAO Wen-bo;LIU Yong-cheng;LIU Si-yu;LI Zhi-xuan;DONGGuo-ju(Xiyuan Hospital of China Academy of Chinese Medical Sciences,Beijing 100091;Graduate School of China Academy of Chinese Medical Sciences,Beijing 100700;National Clinical Research Center for Cardiovascular Diseases of Traditional Chinese Medicine,Beijing 100091)

机构地区：[1]中国中医科学院西苑医院,北京100091 [2]中国中医科学院研究生院,北京100700 [3]国家心血管病临床研究中心,北京100091

出　　处：《世界中西医结合杂志》2024年第3期515-521,527,共8页World Journal of Integrated Traditional and Western Medicine

基　　金：沃华科研基金(BYPDF2211219);国家自然科学基金面上项目(82074423);中国中医科学院科技创新重大攻关项目(CI2021A00903)。

摘　　要：目的观察心可舒片对射血分数保留心力衰竭(Heart failure with preserved ejection fraction,HFpEF)大鼠心脏结构和功能的影响。方法将30只自发性高血压大鼠(Spontaneous hypertension rat,SHR)分为SHR组、HFpEF组、心可舒组(334.8 mg/kg),10只Wistar-Kyoto(WKY)大鼠为对照组。心可舒组给予相应药物灌胃,其余组给予等量生理盐水,连续4周。测量血压和心脏重量指数(Heart mass index,HMI);超声心动图检测大鼠左心房内径(Left atrial diameter,LAD)、室间隔厚度(Interventricular septal thickness,IVST)、左室舒张末期内径(Left ventricular end diastolic diameter,LVEDd)、左室舒张末期后壁厚度(Left ventricular posterior wall thickness,LVPWd)、E/A、E/E′等容舒张时间(Isovolumic relaxation time,IVRT)、左室射血分数(Left ventricular ejection fraction,LVEF);酶联免疫吸附测定法检测血清B型脑尿钠肽(Brain natriuretic peptide,BNP)、血管紧张素Ⅱ(Angiotensin II,Ang II)、醛固酮(Aldosterone,ALD)、可溶性生长刺激表达基因2蛋白(Soluble growth stimulation expressed gene 2 protein,sST2)含量;HE及Masson染色观察心肌组织病理变化。结果与WKY组比较,HFpEF模型组血压升高,HMI增加,差异有统计学意义(P<0.05);LAD、LVEDd、IVST、LVPWd、E/A、E/E′和IVRT增加,差异有统计学意义(P<0.05),LVEF无明显变化,差异无统计学意义(P>0.05);病理观察显示左心房与左心室心肌细胞增大和心肌纤维化;血清BNP、AngⅡ、ALD、sST2含量升高,差异有统计学意义(P<0.05),提示造模成功。与HFpEF模型组比较,心可舒组收缩压降低,HMI减小,差异有统计学意义(P<0.05);LAD、LVEDd、IVST、LVPWd、E/A、E/E′和IVRT减小,差异有统计学意义(P<0.05);病理观察显示心可舒组左心房和心室心肌细胞减小及纤维化改善;血清BNP、AngⅡ、ALD、sST2含量降低,差异有统计学意义(P<0.05)。结论心可舒片能改善HFpEF模型大鼠心房及左心室的结构和功能,其机制可能与调控肾�Objective To observe the effects of the Xinkeshu(XKS)Tablet on cardiac structure and function in rats with heart failure with preserved ejection fraction(HFpEF).Methods Thirty spontaneously hypertensive rats(SHRs)were divided into the SHR group,HFpEF group,and XKS group(334.8 mg/kg),and ten Wistar Kyoto(WKY)rats were used as the control group.The XKS group was given the corresponding drugs by gavage,and the other groups were given the same amount of normal saline for four weeks.Blood pressure and heart mass index(HMI)were measured.Echocardiography was used to detect the left atrial diameter(LAD),interventricular septal thickness(IVST),left ventricular end-diastolic diameter(LVEDd),left ventricular posterior wall thickness(LVWPd),E/A,E/E′,isovolumetric relaxation time(IVRT),and left ventricular ejection fraction(LVEF).The serum levels of brain natriuretic peptide(BNP),angiotensin ar ejection fraction(LVEF thickness(IVST),left ventricular end-diastolic diameter(LVEDd),left ventricular posterior wa immunosorbent assay.HE and Masson staining were used to observe the pathological changes in myocardial tissue.Results Compared with the WKY group,the blood pressure and HMI of the HFpEF model group were increased,and the difference was statistically significant(P<0.05).LAD,LVEDd,IVST,LVPWd,E/A,E/E′,and IVRT were increased(P<0.05),but LVEF did not change significantly,with the difference not statistically significant(P>0.05).Pathological observation showed that the left atrium and left ventricle had enlarged cardiomyocytes and myocardial fibrosis.The serum levels of BNP,AngⅡ,ALD,and sST2 were increased(P<0.05),suggesting that the model was successfully established.Compared with the HFpEF group,systolic blood pressure and HMI were decreased in the XKS group(P<0.05).LAD,LVEDd,IVST,LVPWd,E/A,E/E′,and IVRT were decreased(P<0.05).The pathological observation showed a reduction in cardiomyocytes and an improvement in myocardial fibrosis of the left atrium and ventricle.The serum levels of BNP,AngⅡ,ALD,and sST2 were dec

关 键 词：心可舒片 射血分数保留心力衰竭 HFpEF 

分 类 号：R285.5[医药卫生—中药学]