糖尿病皮肤溃疡与普通皮肤溃疡中炎症蛋白差异性表达的研究  

Differential expression of inflammatory proteins in diabetic skin ulcers and ordinary skin ulcers

作　　者：熊武[1] 贺有缘 张熙 周建大[4] 陈佳[4] 邹晓玲[2] 赵思佳 钟星星 曹雨昙 瞿文静 Xiong Wu;He Youyuan;Zhang Xi;Zhou Jianda;Chen Jia;Zou Xiaoling;Zhao Sijia;Zhong Xingxing;Cao Yutan;Qu Wenjing(Department of Burn and Plastic Surgery,The First Hospital of Hunan University of Chinese Medicine,Changsha 410007,China;Department of Endocrinology,The First Hospital of Hunan University of Chinese Medicine,Changsha 410007,China;School of Clinical Medicine,Hunan University of Chinese Medicine,Hunan Brain Hospital,Changsha 410007,China;Burn and Plastic Surgery Department of Xiangya Third Hospital,Central South University,Changsha 410013,China;College of Integrated Traditional Chinese and Western Medicine,Hunan University of Chinese Medicine,Changsha 410208,China)

机构地区：[1]湖南中医药大学第一附属医院烧伤整形外科,长沙410007 [2]湖南中医药大学第一附属医院内分泌科,长沙410007 [3]湖南中医药大学临床医学院,湖南省脑科医院,长沙410007 [4]中南大学湘雅三医院烧伤整形外科,长沙410013 [5]湖南中医药大学中西医结合学院,长沙410208

出　　处：《中国医师杂志》2024年第3期331-336,共6页Journal of Chinese Physician

基　　金：国家自然科学基金(82374276);湖南省自然科学基金科卫联合项目(2021JJ70033);湖南省卫生健康委科研计划项目(202204034510);湖南省科技厅临床医疗技术创新引导项目(2021SK50802, 2021SK51412)。

摘　　要：目的研究并筛选炎症蛋白在糖尿病皮肤溃疡与普通皮肤溃疡中差异性表达,为深入研究糖尿病皮肤溃疡抗炎促愈药物靶点提供实验依据。方法采集湖南中医药大学第一附属医院11例糖尿病皮肤溃疡患者的组织、12例普通皮肤溃疡患者的组织和11例正常皮肤患者的组织。用酶联免疫吸附(ELISA)法分别检测三组组织中炎症蛋白Toll样受体4(TLR4)、核因子-κB(NF-κB),促炎因子干扰素-γ(IFN-γ)、肿瘤坏死因子-α(TNF-α)、白细胞介素-6(IL-6)、白细胞介素-8(IL-8)、白细胞介素-1β(IL-1β)、巨噬细胞趋化蛋白-1(MCP-1),抗炎因子表皮生长因子(EGF)、白细胞介素-4(IL-4)、白细胞介素-10(IL-10)的含量。结果与正常组织相比,普通溃疡皮肤组织和糖尿病溃疡组织中的TLR4、NF-κB、IFN-γ、TNF-α、IL-1β、IL-6、IL-8、MCP-1和EGF浓度均更高,IL-10浓度更低,差异均具有统计学意义(均P<0.05);与正常组织相比,糖尿病溃疡组织中IL-4浓度更低,差异具有统计学意义(P<0.05);与普通溃疡皮肤组织相比,糖尿病溃疡组织中TLR4、NF-κB和MCP-1浓度更高,IL-4浓度更低,差异均具有统计学意义(均P<0.05)。结论糖尿病患者皮肤溃疡会出现炎症反应,而高糖促进了皮肤溃疡炎症反应,可能与TLR4、NF-κB、MCP-1和IL-4表达异常有关,TLR4/NF-κB信号通路及炎症因子MCP-1和IL-4有望作为糖尿病皮肤溃疡炎症调控的靶点。Objective To study and screen the differential expression of inflammatory proteins in diabetes skin ulcers and common skin ulcers,so as to provide experimental basis for further research on anti-inflammatory and healing drug targets of diabetes skin ulcers.Methods The tissues of 11 patients with diabetes skin ulcer,12 patients with common skin ulcer and 11 patients with normal skin were collected from the First Hospital of Hunan University of Chinese Medicine.The levels of inflammatory protein Toll like receptor 4(TLR4),nuclear factorκB(NF-κB),pro-inflammatory factor interferon-γ(IFN-γ),tumor necrosis factor-α(TNF-α),interleukin-6(IL-6),interleukin-8(IL-8),interleukin-1β(IL-1β),macrophage chemotactic protein-1(MCP-1),anti-inflammatory factors epidermal growth factor(EGF),interleukin-4(IL-4),and interleukin-10(IL-10)were detected in three groups of tissues using enzyme-linked immunosorbent assay(ELISA).Results Compared with normal tissues,the concentrations of TLR4,NF-κB,IFN-γ,TNF-α,IL-1β,IL-6,IL-8,MCP-1 and EGF in common ulcer skin tissues and diabetes ulcer tissues were higher,and the concentrations of IL-10 were lower,with statistically significant differences(all P<0.05);Compared with the normal tissue,the concentration of IL-4 in diabetes ulcer tissue was lower,the difference was statistically significant(P<0.05);Compared with ordinary ulcer skin tissue,the concentrations of TLR4,NF-κB and MCP-1 in diabetes ulcer tissue were higher,and the concentrations of IL-4 were lower,with statistically significant differences(all P<0.05).Conclusions The skin ulcer in diabetes patients will have inflammatory reaction,and high glucose promotes the inflammatory reaction of skin ulcer,which may be related to the abnormal expression of TLR4,NF-κB,MCP-1 and IL-4.TLR4/NF-κB signal pathway and inflammatory factors MCP-1 and IL-4 may be the target of the inflammation regulation of diabetes skin ulcer.

关 键 词：糖尿病 皮肤溃疡 炎症蛋白 促炎因子 抗炎因子 

分 类 号：R587.2[医药卫生—内分泌] R751[医药卫生—内科学]