EDC诱导丝素蛋白制备水稳定性和延展性增强的丝素膜  

EDC Induced Silk Fibroin to Prepare Silk Films with Enhanced Water Stability and Extensibility

作　　者：王鹏伟 刘海宇 胡豆豆 严会超[1] Wang Pengwei;Liu Haiyu;Hu Doudou;Yan Huichao(College of Animal Science,South China Agricultural University,Guangzhou 510642,China)

机构地区：[1]华南农业大学动物科学学院,广州510642

出　　处：《蚕业科学》2024年第1期34-41,共8页ACTA SERICOLOGICA SINICA

基　　金：广东省现代农业产业技术体系创新团队建设项目(2021KJ24)。

摘　　要：为拓展丝素蛋白(silk fibroin, SF)膜在皮肤组织工程中的应用,文中通过制备含有一定比例交联剂1-乙基-3-(3-二甲氨基丙基)碳二亚胺(1-ethyl-3-(3-dimethylamino-propyl) carbodiimide, EDC)的SF水溶液,得到了水稳定性和延展性增强的丝素蛋白膜,并对所得丝素蛋白膜的溶失率、力学性能、交联度和二级结构进行了表征和分析。与纯SF膜(silk fibroin film, SFF)相比,EDC处理的SF膜(SFF-EDC)的溶失率从98.2%大幅下降到6.1%;力学性能方面,按EDC∶SF质量比为10∶100制备的丝素膜SFF-10EDC的断裂伸长率达到170.97%,是SFF的60倍;EDC可引起SF链分子内和分子间酰胺键的形成,促进SF分子构象从无规卷曲转变为β折叠结构。结果表明,在与SF的相互作用中EDC不仅可作为交联剂,还可作为增塑剂。该研究为构建柔性SF膜提供了新方法。In order to expand the application of silk fibroin(SF)films in skin tissue engineering,this study prepared SF aqueous solutions containing a certain proportion of the crosslinking agent 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide(EDC),and obtained SF films with enhanced water stability and extensibility.The study characterized and analyzed the dissolution rate,mechanical properties,degree of crosslinking,and secondary structure of the obtained SF films.Compared to the pure SF films(SFF),the dissolution rate of EDC-treated SF films(SFF-EDC)decreased significantly from 98.2%to 6.1%.In terms of mechanical properties,the fracture elongation of SFF-10EDC prepared with the mass ratio of EDC∶SF being 10∶100 reached 170.97%,which was 60 times higher than that of the SFF.EDC was observed to induce the formation of amide bonds within and between SF molecules,promoting the transformation of SF molecular conformation from random coil toβ-sheet structure.These results suggest that EDC,in addition to acting as a crosslinking agent,also serves as a plasticizer in interaction with SF.This study provides a new method for constructing flexible SF films.

关 键 词：丝素蛋白 交联剂 水稳定性 延展性 薄膜 

分 类 号：R318.08[医药卫生—生物医学工程] S886.9[医药卫生—基础医学]