昆虫杆状病毒多角体蛋白基因超高水平表达的分子机制研究进展  

Research Progress on Molecular Mechanism for the Hyperexpression of Baculovirus Polyhedrin Gene

作　　者：陈官平 李跃东 李佳乐 吴小锋[1,2] Chen Guanping;Li Yuedong;Li Jiale;Wu Xiaofeng(College of Animal Sciences,Zhejiang University,Hangzhou 310058,China;Zhejiang Provincial Key Laboratory of Utilization and Innovation of Silkworm and Bee Resources,Hangzhou 310058,China)

机构地区：[1]浙江大学动物科学学院,杭州310058 [2]浙江省蚕蜂资源利用与创新研究重点实验室,杭州310058

出　　处：《蚕业科学》2024年第1期73-84,共12页ACTA SERICOLOGICA SINICA

基　　金：国家自然科学基金项目(32172793,31972619);国家重点研发计划项目(2022YFE0124400);浙江省自然科学基金项目(LZ20C170001)。

摘　　要：昆虫杆状病毒的生活史中令人惊奇的现象之一是其极晚期基因多角体蛋白(polyhedrin,polh)基因的超高水平表达,表达产生的多角体蛋白将病毒粒子包埋到内部形成包涵体。本文根据已有的研究结果,从3个方面概括了polh基因超高水平表达的机制,即polh启动子的结构特征和转录调控、polh mRNA的结构和翻译调控以及影响polh基因表达的蛋白调控因子,期望有助于更好地理解polh超高水平表达调控的分子机制,为提高杆状病毒表达载体系统表达外源蛋白效率提供理论指导。One of the amazing phenomena in the baculovirus life cycle is the hyperexpression of the very late gene,polyhedrin(polh),causing the production of the occlusion bodies where progeny virions are embedded.In this review,the mechanism responsible for polh hyperexpression from the previous studies was comprehensively summarized from three aspects,namely the structural characteristics of the polh promoter and transcriptional regulation,the structure and translational regulation of the polh mRNA,and especially the regulators that influence the expression of polh,hoping to help better understanding the regulatory molecular mechanism of hyperexpression of polh and provide theoretical guid⁃ance for improving the expression efficiency of foreign proteins by adopting the baculovirus expression vector system.

关 键 词：杆状病毒 多角体蛋白 超高水平表达 分子机制 

分 类 号：S884.51[农业科学—特种经济动物饲养]