桥本甲状腺炎UGRP1与Fas介导的凋亡通路的相关性研究  

Correlation between UGRP1 and apoptosis pathway mediated by Fas in hashimoto thyroiditis

作　　者：马珊珊 吴月 朱莉[1] 陆晨阳 左春林[1] Ma Shanshan;Wu Yue;Zhu Li;Lu Chenyang;Zuo Chunlin(Dept of Endocrinology,The First Affiliated Hospital of Anhui Medical University,Hefei 230022)

机构地区：[1]安徽医科大学第一附属医院内分泌科,合肥230022

出　　处：《安徽医科大学学报》2024年第2期289-292,共4页Acta Universitatis Medicinalis Anhui

基　　金：国家自然科学基金(编号:81270864)。

摘　　要：目的探讨桥本甲状腺炎(HT)中子宫球蛋白相关蛋白1(UGRP1)与Fas介导的凋亡通路的相关性。方法免疫组化(IHC)法检测正常人、HT患者甲状腺细胞UGRP1的表达。在体外用质粒转染大鼠甲状腺细胞(FRTL-5细胞),分别为空载体质粒组、UGRP1质粒组、Fas质粒组,采用实时荧光定量逆转录PCR(RT-qPCR)方法检测各组细胞Fas、UGRP1基因表达水平的变化。结果HT患者甲状腺细胞UGRP1表达呈阳性,正常人甲状腺细胞UGRP1表达呈阴性。转染UGRP1质粒组的Fas基因表达水平较空载体质粒组无明显变化(1.0850±0.1249 vs1.0210±0.1139),转染Fas质粒组的UGRP1基因表达水平较空载体质粒组显著升高(P<0.0001,5.8070±0.3232 vs0.7527±0.0760)。结论桥本甲状腺炎UGRP1高表达可能与Fas介导的凋亡通路相关。Objective To investigate the correlation between uterine globulin associated protein 1(UGRP1) and Fas mediated apoptosis pathway in hashimoto thyroiditis(HT).Methods The expression of UGRP1 in thyroid cells of normal people and HT patients was detected by immunohistochemistry(IHC).FRTL-5 cells were transfected by plasmids in vitro,and control group,UGRP1 group,Fas group were established respectively.Real-time fluorescent quantitative reverse transcription PCR(RT-qPCR) was used to detect the expression of Fas and UGRP1 mRNA in each group.Results UGRP1 expression was positive in thyroid cells of HT patients and negative in that of normal people.There were no significant differences between control group and UGRP1 group in Fas gene expression(1.085 0±0.124 9 vs 1.021 0±0.113 9).Compared with the control group,the expression of UGRP1 gene increased significantly in Fas group(P<0.000 1,5.807 0±0.323 2 vs 0.752 7±0.076 0).Conclusion The high expression of UGRP1 in HT may be related to apoptosis pathway mediated by Fas.

关 键 词：子宫球蛋白相关蛋白1 Fas/FasL通路 桥本甲状腺炎 

分 类 号：R581.4[医药卫生—内分泌]