小花山柰高效快繁技术研究  

Study on Efficient Rapid Propagation Technology of Kaempferia parviflora

作　　者：冯莹 王冰清 殷爱华 唐永锐 何健强 胡淑仪 刘珊 Feng Ying;Wang Bingqing;Yin Aihua;Tang Yongrui;He Jianqiang;Hu Shuyi;Liu Shan(Guangdong Forest Resource Conservation Center,Liwan Guangzhou510173;Foshan Instituteof Forestry(Foshan Botanical Garden),Foshan Guangdong528222)

机构地区：[1]广东省森林资源保育中心,广州荔湾510173 [2]佛山市林业科学研究所(佛山植物园),广东佛山528222

出　　处：《热带林业》2023年第4期41-45,共5页Tropical Forestry

基　　金：广东省林业科技创新项目(2021KJCX002):林下经济植物(山奈属和芋属等)种质资源收集保存、利用与种植示范。

摘　　要：通过研究不同激素配比对小花山柰不定芽的诱导、不定芽增殖及生根的影响,筛选出小花山柰组培快繁最佳的激素配比,建立小花山柰芽基部的高效组培快繁体系。结果表明:小花山柰最佳的不定芽诱导培养基为MS+30g/L蔗糖+7g/L琼脂+2mg/L 6-BA+0.1mg/L NAA;最佳的不定芽增殖培养基为MS+30g/L蔗糖+8g/L琼脂+2mg/L 6-BA+0.5mg/L KT+0.1mg/L NAA;最佳的生根培养基为MS+30g/L蔗糖+7g/L琼脂+0.2mg/L NAA;炼苗移栽15d后成活率达100%,缩短了小花山柰繁殖时间并提升了繁殖系数,降低了小花山柰的育苗成本,为小花山柰组培苗工业化生产提供了技术参考。Using the bud base of Kaempferia parviflora as the experimental material,the optimal hormone ratio for tissue culture and rapid propagation of Kaempferia parviflora was selected by studying the effects of different hormone ratios on the induction,proliferation,and rooting of adventitious buds in Kaempferia parviflora.An efficient tissue culture and rapid propagation system for Kaempferia parviflora was established.The results showed that the optimal medium for inducing adventitious buds in Kaempferia parviflora was MS+30g/L Sucrose+7g/L Agar+2mg/L 6-BA+0.1mg/L NAA;The optimal medium for adventitious bud proliferation is MS+30g/L Sucrose+8g/L Agar+2mg/L 6-BA+0.5mg/L KT+0.1mg/L NAA;The optimal rooting culture medium is MS+30g/L Sucrose+7g/L Agar+0.2mg/L NAA;After 15 days of transplanting and refining seedlings,the survival rate reached 100%,which shortened the propagation time and increased the propagation coefficient of the small flower Kaempferia parviflora reduced the seedling cost of the small flower Kaempferia parviflora,and provided technical reference for the industrial production of the small flower Kaempferia parviflora tissue culture seedlings.

关 键 词：高效快繁 芽基部 小花山柰 

分 类 号：Q949.718.33[生物学—植物学]