参柏舒心颗粒HPLC指纹图谱建立  

HPLC Fingerprint Study of Shenbaishuxin Granules

作　　者：刘爽[1] 王翠竹[2] 李平亚[2] 姜维铮 杜秀娟 LIU Shuang;WANG Cuizhu;LI Pingya;JIANG Weizheng;DU Xiujuan(The First Hospital of Jilin University,Changchun 130000,China;School of Pharmaceutical Sciences,Jilin University,Changchun 130000,China;Jilin Shengya Pharmaceutical Science and Technology Development Co.,Ltd,Dunhua 133700,China)

机构地区：[1]吉林大学第一临床医院,吉林长春130000 [2]吉林大学药学院,吉林长春130000 [3]吉林圣亚医药科技开发有限公司,吉林敦化133700

出　　处：《特产研究》2024年第2期99-102,共4页Special Wild Economic Animal and Plant Research

基　　金：吉林省科技发展计划项目(20190304107YY)。

摘　　要：本文旨在建立参柏舒心颗粒HPLC指纹图谱。本研究采用Waters C18色谱柱(4.6 mm×250 mm,5μm);流动相:乙腈-0.05%三氟乙酸水溶液;流速为0.8 mL/min,进行梯度洗脱;柱温为40℃;检测波长为288 nm。建立10批参柏舒心颗粒HPLC指纹图谱,利用“中药色谱指纹图谱相似度评价系统(2012版)”软件对其进行相似度评价。建立了参柏舒心颗粒HPLC指纹图谱,共确定了25个共有峰,鉴定了5个成分,分别为丹参素钠,原儿茶醛,黄柏碱,丹酚酸B,欧前胡素;10批样品相似度均大于0.93,方法可靠。本研究分析方法具有较强的可行性,达到良好的分离效果,可为提高参柏舒心颗粒生产质量控制提供一定的科学依据。To establish the HPLC fingerprints of Shenbaishuxin granules.The waters C18(4.6 mm 250 mm,5 m)was used,the mobile phase was acetonitrile 0.05%trifluoroacetic acid solution,the flow rate was 1.0 mL/min with gradient elution,the column temperature was 40℃,the detection wavelength was 254 nm.The HPLC fingerprint of ten batches of Shenbaishuxin granules was determined.The similarity evaluation was performed by the"Chinese Herbal Chromatographic Similarity Evaluation System(2012 edition)"software.The HPLC fingerprint of Shenbaishuxin granules was established.A total of 25 common peaks were determined and five component were identified.The similarities of ten samples were higher than 0.93,and the method was reliable.This study the analytical method showd feasibility and good separation effect,providing a scientific basis for improving the quality control of Shenbaishuxin granules.

关 键 词：参柏舒心颗粒 指纹图谱 相似度评价 

分 类 号：R286.0[医药卫生—中药学]