基于叶片解剖结构评价不同倍性橡胶树无性系抗旱性  

Drought Resistance Assessment on Hevea Clones with Different Ploidy Based on Leaf Anatomical Structure

作　　者：邱彦芬 杨湉 吴裕[1] QIU Yanfen;YANG Tian;WU Yu(Yunnan Institute of Tropical Crops/Yunnan Key Laboratory of Sustainable Utilization Research on Rubber Tree/National and Local Joint Engineering Research Center of Breeding and Cultivation Technology of Rubber Tree,Jinghong 666100,China)

机构地区：[1]云南省热带作物科学研究所/云南省天然橡胶可持续利用研究重点实验室(筹)/天然橡胶良种选育与栽培技术国家地方联合工程研究中心,云南景洪666100

出　　处：《热带农业科技》2024年第2期61-67,共7页Tropical Agricultural Science & Technology

基　　金：省所热带作物科技创新专项资金(RF2023-2)。

摘　　要：以12个橡胶树无性系的叶片为材料,采用流式细胞仪和核型分析相结合鉴定倍性,石蜡切片法比较不同倍性植株的叶片解剖结构,最终通过主成分分析与隶属函数法评价其抗旱性水平。结果表明:12个无性系中包含5个三倍体、7个二倍体;两种倍性植株的叶片解剖结构在绝大多数无性系间存在显著差异。通过多重比较和主成分分析,筛选出栅海比、叶片紧密度、叶片厚度、栅栏组织厚度、海绵组织厚度、中脉厚度、下表皮厚度等7项具有代表性的抗旱性评价指标,用隶属函数法得出不同倍性无性系的抗旱排序为187>584>585>272>314>600>186>190>637>620>281>277,无性系187、584、585在生产实际中也具有较强的抗旱性,与上述评价结果相吻合。In this study,12 clonal leaves Heveawere identified by flow cytometry and karyotype analysis.The thickness dif-ference of the two types of leaves was compared by paraffin section to evaluate their drought resistance.The results showed that the 12 clones contained 5 triploids and 7 diploids.The anatomical structure of the two ploidy leaves showed significant differences in most of the clones.Through multiple comparison and principal component analysis,the ratio of gate to sea,leaf density,leaf thickness,palisade tissue thickness and spongy tissue thickness were screened.The seven rep-resentative indexes of midvein thickness and lower epidermis thickness were calculated by membership function method.The drought resistance order of different ploidy clones was 187>584>585>272>314>600>186>190>637>620>281>277.The clones(187,584,585)also have strong drought resistance in practical production,which is consistent with the above evaluation results.

关 键 词：橡胶树 叶片 解剖结构 抗旱性 

分 类 号：S794.101[农业科学—林木遗传育种]