柴胡提取物及其所含皂苷a和皂苷d对小鼠骨骼增长的作用比较  

Comparison of the effects of bupleurum extract,saikosaponin a and saikosaponin d on bone growth in mice

作　　者：梁国辉 靳国强 吕艳艳 谢艳 LIANG Guohui;JIN Guoqiang;LYU Yanyan;XIE Yan(Department V of Hip Joint Surgery,Luoyang Orthopaedic-Traumatological Hospital of He'nan Province(He'nan Provincial Orthopaedic Hospital),Luoyang,He'nan 471002,China)

机构地区：[1]河南省洛阳正骨医院(河南省骨科医院)髋关节外五科,河南洛阳471002

出　　处：《安徽医药》2024年第5期878-882,共5页Anhui Medical and Pharmaceutical Journal

基　　金：河南省中医药科学研究专项课题(20-21ZY1062,20-21ZY2255)。

摘　　要：目的比较柴胡提取物及其所含皂苷a和皂苷d促进骨骼增长的作用及其机制研究。方法自2021年3月至2022年1月进行了高效液相色谱法测定柴胡提取物中柴胡皂苷a与d含量;然后将昆明种小鼠40只采用随机数字表法分为健康对照组、柴胡皂苷a组(简称皂苷a组)、柴胡皂苷d组(简称皂苷d组)、柴胡提取物组(简称提取物组)和阳性对照组,每组8只。健康对照组灌胃去离子水,皂苷a组灌胃柴胡皂苷a 1.15μg/g体质量,皂苷d组灌胃柴胡皂苷d 0.2μg/g体质量,柴胡提取物组按照0.94 mg/g体质量灌胃,阳性对照组灌胃酸枣仁皂苷a0.013 mg/g体质量灌胃。灌胃25 d后于末次给药30 min后取脑组织,蛋白质印迹法检测脑组织色氨酸羟化酶2(TPH2)含量,ELISA法检测脑组织5-羟色胺(5-HT)、生长激素(GH)含量的变化和5-HT与5-羟色胺1A受体(5-HT1AR)结合活性,并测量各组药物对小鼠胫骨长度的影响。结果柴胡提取物中柴胡皂苷a含量为1.22 mg/g,柴胡皂苷d含量为0.22 mg/g;提取物组和皂苷a组的脑组织5-HT含量(60.93±11.09,59.51±11.02)μg/L、GH含量(68.91±9.10,68.42±9.03)μg/L、5-HT1AR与5-HT结合活性(39.90±8.36,37.27±6.59)及胫骨长度(10.56±0.53,10.55±0.54)cm均较健康对照组[(40.89±8.74)μg/L,(44.87±8.92)μg/L,27.34±3.45,(9.48±0.63)cm]明显增高(P<0.01),同时这两组TPH2含量较健康对照组升高(P<0.01),均差异有统计学意义。皂苷d组脑组织TPH2蛋白含量、5-HT含量(47.46±8.62)、GH含量(45.81±7.14)、5-HT1AR与5-HT结合活性(30.87±5.52)及胫骨长度(9.88±0.56)cm较健康对照组差异无统计学意义(P>0.05)。与皂苷a组比较,皂苷d组的脑组织TPH2蛋白含量、5-HT含量、5-HT1AR与5-HT结合活性及胫骨长度减少(P<0.05),GH含量明显减少(P<0.01),差异有统计学意义。与皂苷d组比较,提取物组GH含量、5-HT1AR与5-HT结合活性及胫骨长度增加明显增高(P<0.01),5-HT含量增高(P<0.05),差异有统计学意义。结论柴胡�Objective To compare the role and mechanism of bupleurum extract,saikosaponin a and saikosaponin d in promoting bone growth.Methods The contents of saikosaponin a and d in bupleurum extract were determined by high-performance liquid chro⁃matography from March 2021 to January 2022.Forty Kunming strain mice were randomly assigned into normal control group,saikosa⁃ponin a group(referred to as saponin a group),saikosaponin d group(referred to as saponin d group),bupleurum extract group(referred to as extract group)and positive control group.Normal control group received gavage with deionized water,saponin a group with saiko⁃saponin a 1.15μg/g body weight,saponin d group gavage saikosaponin d 0.2μg/g body weight,bupleurum extract group 0.94 mg/g body weight,and positive control group acid jujube kernel saponin a 0.013 mg/g body weight.After 25 days of gavage,brain tissue of mice was taken 30 minutes after the last dose,and western blotting was used to detect the content of tryptophan hydroxylase 2(TPH2)in brain tissue,and ELISA was used to detect the change of 5-hydroxytryptamine(5-HT)and growth hormone(GH)in brain tissue,and the binding activity of 5-HT and 5-hydroxytryptamine 1A receptor(5-HT1AR),and the effect of drugs in each group on the tibia length of mice was also measured.Results The contents of saikosaponin a in the extract was 1.22 mg/g,while that of saikosaponin d was 0.22 mg/g.The 5-HT and GH contents of brain tissue,binding activity of 5-HT1AR and 5-HT and tibial length in the extract group and the saponin a group were significantly increased[(60.93±11.09,59.51±11.02)μg/L,(68.91±9.10,68.42±9.03)μg/L,(39.90±8.36,37.27±6.59)and(10.56±0.53,10.55±0.54)cm]in comparison with those in normal control group[(40.89±8.74)μg/L,(44.87±8.92)μg/L,(27.34±3.45),and(9.48±0.63)cm](P<0.01).Meanwhile,the contents of TPH2 in these two groups were also increased in comparison with normal control group(P<0.01),and differences were found to be significant.The contents of TPH2,5-HT(47.46±8.62),and GH(45.8

关 键 词：柴胡属 柴胡皂苷A 柴胡皂苷D 骨骼增长 

分 类 号：R285.5[医药卫生—中药学]