Advances in In Vitro and In Vivo Bioreactor-Based Bone Generation for Craniofacial Tissue Engineering  

作　　者：Emma Watson Antonios G.Mikos 

机构地区：[1]Department of Bioengineering,Rice University,Houston,TX 77030,USA

出　　处：《Biomedical Engineering Frontiers》2023年第1期203-216,共14页生物医学工程前沿（英文）

基　　金：This work was supported,in part,by the U.S.Army,Navy,NIH,Air Force,and Veterans Affairs and Health Affairs to support the Armed Forces in Regenerative Medicine,under Award W81XWH-14-2-0004;This work was also supported by the Osteo Science Foundation and the NIH(P41 EB023833);E.W.was supported by the National Institute of Dental and Craniofacial Research(F31 DE027586).

摘　　要：Craniofacial reconstruction requires robust bone of specified geometry for the repair to be both functional and aesthetic.While native bone from elsewhere in the body can be harvested,shaped,and implanted within a defect,using either an in vitro or in vivo bioreactors eliminates donor site morbidity while increasing the customizability of the generated tissue.In vitro bioreactors utilize cells harvested from the patient,a scaffold,and a device to increase mass transfer of nutrients,oxygen,and waste,allowing for generation of larger viable tissues.In vivo bioreactors utilize the patient’s own body as a source of cells and of nutrient transfer and involve the implantation of a scaffold with or without growth factors adjacent to vasculature,followed by the eventual transfer of vascularized,mineralized tissue to the defect site.Several different models of in vitro bioreactors exist,and several different implantation sites have been successfully utilized for in vivo tissue generation and defect repair in humans.In this review,we discuss the specifics of each bioreactor strategy,as well as the advantages and disadvantages of each and the future directions for the engineering of bony tissues for craniofacial defect repair.

关 键 词：DEFECT IMPLANTATION utilize 

分 类 号：R318[医药卫生—生物医学工程]