一株肉种鸡源鸭疫里默氏杆菌分离鉴定及致病性研究  

Identification and Pathogenic Analysis on a Strain of Riemerella anatipestifer Isolated from Broiler Breeder

作　　者：汪建华 马芹 王胜 刘丹丹 许明清 赵杰 张中波 邹志强 刘子涵 魏书强 李玉峰[2] WANG Jianhua;MA Qin;WANG Sheng;LIU Dandan;XU Mingqing;ZHAO Jie;ZHANG Zhongbo;ZOU Zhiqiang;LIU Zihan;WEI Shuqiang;LI Yufeng(Shandong Hekangyuan Biological Breeding Co.,Ltd.,Ji'nan,Shandong 250001;Poultry Institute of Shandong Academy of Agricultural Sciences,Ji'nan,Shandong 250023)

机构地区：[1]山东和康源生物育种股份有限公司,山东济南250001 [2]山东省农业科学院家禽研究所,山东济南250023

出　　处：《中国家禽》2024年第4期107-112,共6页China Poultry

基　　金：山东省农业科学院农业科技创新工程(CXGC2021B22)。

摘　　要：为探究鸭疫里默氏杆菌对鸡的致病力,试验采集疑似感染鸭疫里默氏杆菌的病死肉种鸡肝脏进行病原的分离培养与纯化、革兰氏染色、生化试验、血清型鉴定、16S rRNA基因测序分析及药敏试验,并将分离菌株在SPF鸡、樱桃谷鸭上进行致病性试验。结果显示:分离到1株血清1型鸭疫里默氏杆菌,该菌株与鸭源、鸡源、鹅源鸭疫里默氏杆菌的相似性分别为99.24%~99.47%、99.16%~99.31%、99.04%~99.20%;该分离株对恩诺沙星、卡那霉素、头孢噻肟、多黏菌素B和多西环素较为敏感,对SPF鸡致病性较弱,可引起一过性精神沉郁和泡沫粪,对樱桃谷鸭有较强的致病性,可引起死亡和严重的纤维素性心包炎、肝周炎和气囊炎。研究表明分离的肉种鸡源RA对鸡致病力相对较弱,结果为临床鸡群感染RA的诊断与防控提供参考。In order to explore the pathogenicity of Riemerella anatipestifer(RA)to chicken,the pathogen was isolated and cultured from the liver of diseased broiler breeder suspected infected with RA.The bacterial culture and purification,Gram stain⁃ing,biochemical test,serotype identification,16S rRNA gene sequencing analysis and drug susceptibility test of the isolated pathogen were conducted.The SPF chicken and Cherry Valley duck were used for pathogenicity experiment.The results showed that a strain of serotype 1 RA was isolated,the similarity of which with RA isolated form duck,chicken and goose were 99.24%-99.47%,99.16%-99.31%and 99.04%-99.20%,respectivley.The isolate was more sensitive to enrofloxacin,kanamycin,cefotaxime,polymyxin B,and doxycycline,which had weak pathogenicity to SPF chicken and could cause transient mental depression and foamy feces,which had strong pathogenicity to Cherry Valley duck and could cause death and severe fibrinous pericarditis,perihepatitis and air sacculitis.It's suggested that the pathogenicity of the isolated RA from broiler breeder was relatively weak to chicken,which provided a reference for diagnosis and control of RA infection in chicken.

关 键 词：肉种鸡 血清1型鸭疫里默氏杆菌 分离鉴定 致病性 

分 类 号：S855.1[农业科学—临床兽医学]