基于巢式PCR的松材线虫快速检测体系的建立与应用  

Establishment and Application of Rapid Detection System for Pine Wood Nematode Based on Nested PCR

作　　者：刘广川 吴浩 LIU Guang-chuan;WU Hao(Sichuan Forestry and Grassland Inventory and Planning Institute,Chengdu 610081,China)

机构地区：[1]四川省林业和草原调查规划院,四川成都610081

出　　处：《四川林勘设计》2024年第1期65-74,共10页Sichuan Forestry Exploration and Design

摘　　要：松材线虫病又被称为“松树的癌症”,松木一旦感染,将迅速枯死,最终导致整片松林毁于一旦,对松木资源和生态环境的保护构成了严重威胁。该病防治难度很大,目前尚无良好的防治方法。快速发现并确定松材线虫病情有利于各地林业局及时有效地确定疫区,迅速开展一系列防治措施,以防止因发现过晚而造成的不可挽回态势。本实验研究所建立的巢式PCR检测体系,有着特异性强、准确率高、灵敏度高和成本低等特点,可以在发病早期检测出松材线虫的存在,更早地对感病松树进行处理,值得进行推广。Pine wilt disease are also known as the“cancer of pine trees”,will cause the death of the whole pine forest once the pine trees are infected,causing serious damage to the pine resources and ecological environment.The control of this disease is very difficult and there is no good control method at present.The rapid detection and determination of this disease is beneficial to the local forestry bureaus in determining the epidemic area in a timely and effective manner,and quickly implementing a series of prevention and control measures to prevent the irreversible situation caused by late detection.The two detection systems of nested PCR and qPCR established in this study had the characteristics of strong specificity,high accuracy,high sensitivity and low cost,which can detect the existence of pine wood nematodes in the early stage of disease and treat infected pine trees earlier,which is worthy of promotion.

关 键 词：松材线虫 类甜蛋白基因Bx-TLP-1 巢式PCR 快速检测 

分 类 号：S43[农业科学—农业昆虫与害虫防治]