活血消异方对子宫内膜异位症大鼠卵巢功能及妊娠结局的影响研究  

Study on effect of Huoxue Xiaoyi Formula on ovarian function and pregnancy outcome in rats with endometriosis

作　　者：时光 杨新春 崔景润 张玉 赵瑞华[1] SHI Guang;YANG Xinchun;CUI Jingrun;ZHANG Yu;ZHAO Ruihua(Department of Gynecology,Guang′anmen Hospital,China Academy of Chinese Medical Sciences,Beijing 100053,China;Department of Cardiovascular,Guang′anmen Hospital,China Academy of Chinese Medical Sciences,Beijing 100053,China;Graduate School of People′s Liberation Army Medical College,Beijing 100853,China)

机构地区：[1]中国中医科学院广安门医院妇科,北京100053 [2]中国中医科学院广安门医院心血管科 [3]解放军医学院研究生院

出　　处：《北京中医药大学学报》2024年第3期374-382,共9页Journal of Beijing University of Traditional Chinese Medicine

基　　金：国家自然科学基金面上项目(No.82074485);中国中医科学院科技创新工程重点攻关项目(No.CI2021A02403)。

摘　　要：目的 探讨活血消异方对子宫内膜异位症大鼠卵巢功能及妊娠结局的影响。方法 100只雌性SD大鼠适应性喂养7 d,取动情周期正常的大鼠98只,按照体质量随机分为空白组(n=14)、假手术组(n=14)、供体组(n=12)、受体组(n=58)。应用同种异体子宫内膜移植法建立子宫内膜异位症大鼠模型,造模成功的56只受体组大鼠按照体质量随机分为模型组及活血消异方低、中、高剂量组,每组14只。活血消异方低、中、高剂量组分别予5.86、11.72、23.44 g/kg活血消异方灌胃,空白组、假手术组、模型组予蒸馏水灌胃,连续15 d。每组按体质量随机选取6只大鼠取腹主动脉血,酶联免疫吸附测定法检测血清抗米勒管激素(AMH)含量;剩余大鼠每组按体质量随机选取6只行高频小动物超声检查,测定卵巢体积、卵巢动脉血供(阻力指数和搏动指数),并行卵泡计数。雌鼠与雄鼠以2∶1合笼,观察妊娠率、活胎数、死胎数、吸收胎数,测量子宫连胎质量,测量活胎胎盘质量、胎鼠质量、身长、尾长,并对活胎进行骨骼及内脏畸形鉴定。结果 各组大鼠血清AMH含量、卵巢体积比较差异无统计学意义。与模型组比较,空白组,假手术组,活血消异方低、中、高剂量组大鼠卵泡计数均增加;与活血消异方低剂量组比较,空白组、活血消异方中剂量组大鼠卵泡数目均增加(P<0.05)。与模型组比较,空白组,假手术组,活血消异方低、中、高剂量组卵巢动脉血流阻力指数与搏动指数均降低(P<0.05)。各组间大鼠妊娠率比较差异无统计学意义。与模型组、活血消异方低剂量组比较,空白组,假手术组,活血消异方中、高剂量组活胎数和子宫连胎质量均明显增加(P<0.05)。各组间死胎数、吸收胎数,以及各组活胎胎盘质量、胎鼠质量、身长、尾长比较差异无统计学意义。各组活胎胎鼠均未见内脏、骨骼畸形。结论 子宫内膜异位症模型�Objective To explore the effect of Huoxue Xiaoyi Formula on ovarian function and pregnancy outcome in endometriosis rats.Methods One hundred female SD rats were fed adaptively for 7 days.According to body weight,98 rats with a normal estrous cycle were randomly divided into the blank group(n=14),the sham operation group(n=14),the donor group(n=12),and the receptor group(n=58).A rat model of endometriosis was established using allogeneic endometrial transplantation.After the model was successfully established,according to the body weight,56 rats in the receptor group were randomly divided into the model group,and the Huoxue Xiaoyi Formula low⁃,medium⁃,and high⁃dose groups,with 14 rats in each group.The Huoxue Xiaoyi Formula low⁃,medium⁃,and high⁃dose groups were given 5.86,11.72,and 23.44 g/kg of Huoxue Xiaoyi Formula,and the blank group,the sham operation group,and the model group were given distilled water by gavage for 15 consecutive days.According to body weight,six rats were randomly selected to take abdominal aortic blood for determination of serum anti⁃Mullerian hormone(AMH)content by enzyme⁃linked immunosorbent assay.According to body weight,six rats were randomly selected in the remaining rats to undergo high⁃frequency small animal ultrasound examination to measure ovarian volume and ovarian artery blood supply(resistance index and pulsatility index),and follicle count were measured.The female mice were fed with male mice at a ratio of 2∶1 to observe the pregnancy rate,live fetus number,still fetus number,and absorbed fetus number.The weights of uterus and fetus were measured,while the placental weight,fetal weight,body length,and tail length were measured for live fetus.Bone and visceral malformations were also detected for live fetus.Results There were no significant differences in serum AMH and ovarian volume among the groups.Compared with the model group,the numbers of follicles in the blank group,the sham operation group,and the Huoxue Xiaoyi Formula low⁃,medium⁃,and high⁃do

关 键 词：活血消异方 子宫内膜异位症 卵巢功能 妊娠结局 大鼠 

分 类 号：R285.5[医药卫生—中药学]