过表达杜仲EuDIR1基因对番茄灰霉病的抗性影响研究  

Research on the Effect of Overexpression Eucommia ulmoides EuDIR1 Gene on Tomato Resistance to Botrytis cinerea

作　　者：徐艳 杨金玉 陈炳竹 赵德刚[1,2,3,4] 赵懿琛 XU Yan;YANG Jinyu;CHEN Bingzhu;ZHAO Degang;ZHAO Yichen(The Key Laboratory of Ministry of Education for Plant Resources Conservation and Germplasm Innovation in Mountainous Region,College of Tea Sciences and College of Life Sciences,Guizhou University,Guiyang 550025,China;Guizhou Key Lab of Agro-Bioengineering,Guiyang 550025,China;National-Local Joint Engineering Research Center of Karst Region Plant Resources Utilization&Breeding(Guizhou),Guiyang 550025,China;Plants Conservation Technology Center,Guizhou Key Laboratory of Agricultural Biotechnology,Guizhou Academy of Agricultural Sciences,Guiyang 550025,China)

机构地区：[1]贵州大学生命科学学院/茶学院/山地植物资源保护与种质创新教育部重点实验室,贵阳550025 [2]贵州省农业生物工程重点实验室,贵阳550025 [3]喀斯特山区植物资源利用与育种国家地方联合工程研究中心(贵州),贵阳550025 [4]贵州省农业科学院农业生物技术重点实验室/植物保育技术应用工程研究中心,贵阳550025

出　　处：《种子》2024年第2期10-17,29,共9页Seed

基　　金：中央引导地方科技发展资金(黔科合中引地[2023]009);国家自然科学基金(31870285,31660076);贵州省农业科学院人才专项([2022]02号);贵州省人才办贵州省特色植物种质资源利用与创新人才基地(RCJD2018-14)。

摘　　要：Dirigent(DIR)蛋白在木质素的合成中发挥重要作用,同时还能提高植物对各种病原体的抗性。本研究以克隆的杜仲Dirigent1基因(EuDIR1)为基础,构建EuDIR1过表达载体遗传转化番茄,获得转基因植株后分析EuDIR1基因在番茄抗灰霉病中的作用。结果表明,与野生型和转空载番茄相比,接种灰霉菌后过表达EuDIR1基因的番茄发病更晚,病斑直径显著小于野生型与转空载植株,说明EuDIR1显著提高了番茄植株对灰霉病的抗性。过表达EuDIR1基因还可提高番茄植株接菌前后的保护酶活性与番茄病程相关蛋白(PRs)的基因表达,从而增强植株对灰霉病的抗性。此外,EuDIR1基因过表达后番茄叶片和茎的木质素含量都显著增加,且木质部导管附近的细胞壁显著增厚,推测EuDIR1基因可能通过增加番茄中木质素的含量,增厚番茄木质部导管附近的细胞壁来增强番茄对灰霉病的抗性。本研究揭示EuDIR1基因在番茄抗灰霉病中的功能,可为番茄抗病育种提供理论依据和利用途径。Dirigent(DIR)proteins play an important role in the synthesis of lignin,which improve plant resistance to various pathogens.In this study,based on the Dirigent1 gene of Eucommia ulmoides(EuDIR1)cloned in the laboratory,the EuDIR1 overexpression vector was constructed and transformed into tomato.After obtaining the transgenic plants,the role of EuDIR1 gene in tomato resistance to Botrytis cinerea was analyzed.The results showed that compared with wild type and unloaded tomato,tomato with overexpression of EuDIR1 gene after inoculation with Botrytis cinerea occurred later,and the lesion diameter was significantly smaller than that of wild type and and plants transferred into the empty vector,indicating that EuDIR1 significantly improved the resistance of tomato plants to Botrytis cinerea.Overexpression of EuDIR1 gene could also increase the protective enzyme activity and the expression of tomato course-related protein(PRs)gene before and after inoculation,thus enhancing the resistance of tomato plants to Botrytis cinerea.In addition,after overexpression of EuDIR1 gene,the lignin content in tomato leaves and stems increased significantly,and the cell wall near the xylem vessel significantly thickened.It was speculated that EuDIR1 gene could enhance tomato resistance to Botrytis cinerea by increasing lignin content in tomato and thickening the cell wall near xylem vessel.This study revealed the function of EuDIR1 gene in tomato resistance to Botrytis cinerea,which could provide some theoretical basis for tomato disease resistance breeding.

关 键 词：EuDIR1基因 番茄 灰霉病 木质素 抗病 

分 类 号：S641.2[农业科学—蔬菜学]