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作 者:刘玥辰 金昆 薛焱 LIU Yuechen;JIN Kun;XUE Yan(School of Pharmacy,Inner Mongolia Medical University,Hohhot 010110,China)
出 处:《种子》2024年第2期60-65,79,共7页Seed
基 金:国家自然科学基金(81641107);内蒙古高等学校科学研究重点项目(NJZZ22647);内蒙古自然科学基金面上项目(2022MS08014);内蒙古医科大学博士基金项目(YKD2020BSJJ013);内蒙古医科大学面上项目(YKD2023MS083)。
摘 要:为了探究外源激素吲哚乙酸(IAA)和赤霉素(GA_(3))对盐碱胁迫下蒙药材阿给(冷蒿)种子萌发的影响,本实验通过不同浓度NaCl、NaHCO_(3)模拟盐碱胁迫,筛选出与对照相比能使50%冷蒿种子萌发的NaCl和NaHCO_(3)浓度(分别为210 mmol/L和95 mmol/L),研究在此浓度盐碱胁迫下,分别施加不同浓度IAA和GA_(3)对种子萌发的影响。结果表明,随着NaCl、NaHCO_(3)浓度逐渐升高,冷蒿种子发芽率降低。不同浓度IAA和GA_(3)对冷蒿种子发芽率、发芽势、发芽指数具有不同程度的促进作用,且各处理间各指标均存在显著差异,NaCl胁迫下,50 mg/L IAA和80 mg/L GA_(3)处理能显著提高冷蒿种子各萌发指标;NaHCO_(3)胁迫下,50 mg/L IAA和10 mg/L GA_(3)处理能显著提高种子发芽率,说明一定浓度的外源激素对盐碱胁迫下冷蒿种子萌发有促进作用。In order to explore the effects of exogenous hormones indole-3-acetic acid(IAA)and gibberellin(GA_(3))on seed germination of the Inner Mongolia medicinal herb Agi Artemisia frigida Willd.under saline-alkali stress,in this experiment,the concentrations of NaCl and NaHCO_(3) that could make 50%of Artemisia frigida Willd.seeds germinate were screened by simulating saline-alkali stress at different concentrations of NaCl and NaHCO_(3),and the concentrations were 210 mmol/L and 95 mmol/L,under this concentration of saline-alkali stress,different concentrations IAA and effects of GA_(3) on seed germination were studied.The results showed that with the gradual increase of NaCl and NaHCO_(3) concentrations,the germination rate of Artemisia frigida Willd.seeds decreased.Different concentrations of IAA and GA_(3) had different degrees of promotion effect on germination rate,germination potential and germination index of Artemisia frigida Willd.seeds,and there were significant differences in the indexes between the treatments,and under NaCl stress,50 mg/L IAA and 80 mg/L GA_(3) treatment could significantly improve the germination index of Artemisia frigida Willd.seeds.Under NaHCO_(3) stress,50 mg/L IAA and 10 mg/L GA_(3) treatment significantly increased seed germination rate,indicating that a certain concentration of exogenous hormones had a promoting effect on seed germination of Artemisia frigida Willd.under saline-alkali stress.
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