大麦芽碱对B16细胞黑色素合成的影响及其机制研究  

Effect of hordenine on melanin synthesis in B16 cells and mechanism research

作　　者：吴莉 靖申松 夏晶[1] 王雄[1] 孟军华[1] 安靖 吴金虎[1] 邹吉利[1] 陈永刚 WU Li;JING Shensong;XIA Jing;WANG Xiong;MENG Junhua;AN Jing;WU Jinhu;ZOU Jili;CHEN Yonggang(Department of Pharmacy,Wuhan Third Hospital/Tongren Hospital Affiliated to Wuhan University,Hubei Wuhan 430065,China;Wuhan Center of Clinical Laboratory,Hubei Wuhan 430065,China;Department of Pharmacy,People’s Hospital of Macheng City,Hubei Macheng 438300,China)

机构地区：[1]武汉市第三医院/武汉大学附属同仁医院药学部,湖北武汉430065 [2]武汉市临床检验中心,湖北武汉430065 [3]麻城市人民医院药剂科,湖北麻城438300

出　　处：《中国医院药学杂志》2024年第5期545-549,569,共6页Chinese Journal of Hospital Pharmacy

基　　金：武汉市卫生计生委科研计划资助项目(编号:WZ22B03、WZ22M01)。

摘　　要：目的:探讨大麦芽碱对B16细胞黑色素合成过程的影响及其作用机制。方法:Western blot检测大麦芽碱(50,100,200μmol·L^(-1))处理B16细胞后TYR、TRP1、TRP2的表达,判断对黑色素合成过程的影响;通过PKA激动剂处理细胞,研究大麦芽碱对B16细胞黑色素合成过程的影响与cAMP/PKA/CREB/MITF信号通路的关系。结果:大麦芽碱(50,100,200μmol·L^(-1))可以抑制B16细胞黑色素组cAMP、PKA、P-CREB、MITF蛋白的表达,从而抑制黑色素合成过程中TYR、TRP1、TRP2的表达。结论:大麦芽碱可以抑制B16细胞黑色素的合成过程,其机制可能与抑制cAMP/PKA/CREB/MITF信号通路有关。OBJECTIVE To study the effect of hordenine on melanin synthesis process in B16 cells and its mechanism of action.METHODS Western blot was used to detect the expression of TYR,TRP1 and TRP2 in B16 cells after the treatment with hordenine(50,100,200μmol·L^(-1))to determine the impact on the melanin synthesis process.After PKA agonist treatment,the correlation between the effect of hordenine on melanin synthesis process in B16 cells and cAMP/PKA/CREB/MITF signaling pathways.RESULTS Hordenine(50,100,200μmol·L^(-1))could inhibit the protein expression of cAMP,PKA,p-CREB and MITF in melanoma B16 cells group,thus inhibiting TYR,TRP1 and TRP2 expression during melanin synthesis process.CONCLUSION Hordenine can inhibit melanin synthesis process in B16 cells,and the mechanism may be related to inhibiting cAMP/PKA/CREB/MITF signaling pathways.

关 键 词：大麦芽碱 黑色素 B16细胞 CAMP 

分 类 号：R282[医药卫生—中药学]