Development of a cell-permeable adenine-derived probe for capture of nucleotide-binding proteins in living cells  

作　　者：Lihong Liu Rui Chen Gang Xue Chenzhou Hao Weizhi Weng Zhengying Pan 

机构地区：[1]State Key Laboratory of Chemical Oncogenomics,School of Chemical Biology and Biotechnology,Peking University Shenzhen Graduate School,Shenzhen 518055,China

出　　处：《Chinese Chemical Letters》2024年第3期243-246,共4页中国化学快报（英文版）

基　　金：funding support from the National Natural Science Foundation of China(No.81872749);Shenzhen Science and Technology Innovation Commission(Nos.20200815000631001 and JCYJ20200109120414564);Peking University。

摘　　要：Adenine is an essential building block of genetic material and a range of coenzymes.Chemical probes containing an adenine moiety have been used in kinase profiling studies in cell lysates.Here we report that adenine-derived small-molecule probes with an activated ester reactive group can covalently modify a conserved lysine residue of protein kinases and capture a number of nucleotide-binding proteins within living cells.

关 键 词：Adenine-derived probe Cell-permeable Lysine residue Protein kinases Nucleotide-binding proteins 

分 类 号：O657.3[理学—分析化学] Q503[理学—化学]