Applying dynamic light scattering to investigate the self-assembly process of DNA nanostructures  

作　　者：Wei Yuan Gui-Zhi Dong Hui Ning Xiang-Xiang Guan Jia-Feng Cheng Zi-Wei Shi Xiu-Ji Du Si-Wen Meng Dong-Sheng Liu Yuan-Chen Dong 

机构地区：[1]CAS Key Laboratory of Colloid,Interface and Chemical Thermodynamics,Institute of Chemistry,Chinese Academy of Sciences,Beijing 100190,China [2]University of Chinese Academy of Sciences,Beijing 100049,China [3]Dandong Bettersize Instruments Ltd.,Dandong 118000,China [4]Key Laboratory of Organic Optoelectronics&Molecular Engineering of the Ministry of Education,Department of Chemistry,Tsinghua University,Beijing 100084,China [5]Department of Chemistry,Renmin University of China,Beijing 100872,China

出　　处：《Chinese Chemical Letters》2024年第3期316-320,共5页中国化学快报（英文版）

基　　金：supported by the National Natural Science Foundation of China(No.21971248)。

摘　　要：Understanding the dynamic assembly process of DNA nanostructures is important for developing novel strategy to design and construct functional devices.In this work,temperature-controlled dynamic light scattering(DLS)strategy has been applied to study the global assembly process of DNA origami and DNA bricks.Through the temperature dependent size and intensity profiles,the self-assembly process of various DNA nanostructures with different morphologies have been well-studied and the temperature transition ranges could be observed.Taking advantage of the DLS information,rapid preparation of the DNA origami and the brick assembly has been realized through a constant temperature annealing.Our results demonstrate that the DLS-based strategy provides a convenient and robust tool to study the dynamic process of forming hieratical DNA structures,which will benefit understanding the mechanism of self-assembly of DNA nanostructures.

关 键 词：Dynamic light scattering Self-assembly process DNA nanostructures Temperature transition ranges Rapid preparation 

分 类 号：Q75[生物学—分子生物学] TB383.1[一般工业技术—材料科学与工程]