葡萄风信子愈伤组织再生体系的构建  

Construction of Callus Regeneration System in Grape Hyacinth

作　　者：曹晓云 田淑婷 时春莹 谢靖雯 黎敏婕 杜灵娟 Cao Xiaoyun;Tian Shuting;Shi Chunying;Xie Jingwen;Li Minjie;Du Lingjuan(College of Landscape Architecture and Art,Northwest A&F University,Yangling Shaanxi 712100,China)

机构地区：[1]西北农林科技大学风景园林艺术学院,陕西杨凌712100

出　　处：《西南林业大学学报（自然科学）》2024年第2期20-26,共7页Journal of Southwest Forestry University：Natural Sciences

基　　金：国家自然科学基金项目(32171863,31700625)资助。

摘　　要：以葡萄风信子常见种亚美尼亚的花蕾和叶片作为外植体,开展不同因素对葡萄风信子愈伤组织诱导和植株再生影响的研究。结果表明:亚美尼亚的花蕾和叶片愈伤组织诱导和增殖的最佳培养基配方为:MS+1.0 mg/L 2,4−D+0.1 mg/L 6−BA,其愈伤组织诱导率为100%,且继代35 d后不同培养基上的花源和叶源愈伤组织的鲜质量无显著差异;液体培养和光照培养有利于愈伤组织细胞增殖,1 g亚美尼亚花源和叶源愈伤组织经液体培养4个继代周期后,平均鲜质量最高可达(10.36±1.13)g和(10.55±2.29)g,表明不同培养基配方对亚美尼亚花蕾和叶片愈伤组织细胞增殖无显著影响,且液体培养和光照培养均有利于愈伤组织的增殖;胚性和非胚性2种愈伤组织分别通过体细胞胚发生和器官发生2种途径可再生形成完整植株,再生率均为100%。The callus induction and plant regeneration of grape hyacinth was carried out,utilizing the flower buds and leaves of Muscari armeniacum as explants.The results show that the best medium for callus induction and proliferation was MS+1.0 mg/L 2,4-D+0.1 mg/L 6-BA,and the callus induction rate was 100%.After 35 days of subculture,there was no significant difference on callus proliferation in different medium formulations.Meanwhile,liquid culture and light culture were beneficial to the proliferation of calli.After 4 cycles of the subculture of 1 g M.armeniacum flower and leaf-derived callus,the maximum average fresh weight was 10.36±1.13 g and 10.55±2.29 g.The results showed that different media formulations had no significant effect on flower and leaf-derived callus proliferation in M.armeniacum,and both liquid culture and light culture were beneficial to callus proliferation.Furthermore,embryogenic and non-embryogenic callus could be regenerated to plants by somatic embryogenesis and organogenesis,respectively.The regeneration rates were all 100%.

关 键 词：葡萄风信子 愈伤组织 液体培养 器官发生 体细胞胚发生 

分 类 号：S682[农业科学—观赏园艺]