基于转录组测序对不同月龄舍饲滩羊羔羊肌肉脂肪代谢相关基因差异表达分析  

Differential expression analysis of genes related to muscle fat metabolism in different month-old house-feeding Tan lambs based on transcriptome sequencing

作　　者：康燕 王小伟 杜佳文 张宁[1] 李海波[1] 徐晓锋[1] 张力莉[1] KANG Yan;WANG Xiao-wei;DU Jia-wen;ZHANG Ning;LI Hai-bo;XU Xiao-feng;ZHANG Li-li

机构地区：[1]宁夏大学动物科技学院,宁夏银川750021

出　　处：《饲料研究》2024年第5期60-65,共6页Feed Research

基　　金：国家自然科学基金项目“日粮琥珀酸调控滩羊瘤胃菌群代谢及肉质性状的作用与机制(项目编号:32160797)”。

摘　　要：试验旨在探究在舍饲模式下不同月龄滩羊羔羊的肌肉组织脂肪代谢的相关差异表达基因。将体况相近的12只舍饲滩羊为研究对象,分别于2月龄和6月龄挑选3只体重相近的滩羊进行屠宰,取背最长肌和股二头肌。采用Illumina对背最长肌和股二头肌进行转录组测序,筛选脂肪代谢相关基因。结果显示,6月龄与2月龄滩羊羔羊股二头肌共筛选出差异表达基因146个,其中69个上调基因,77个下调基因。差异表达基因富集在40个GO条目中,主要在内皮细胞凋亡过程的调节、羧酸代谢过程及上皮细胞凋亡过程的负向调节等生物过程中。KEGG显著富集在4个通路中,与脂肪代谢密切相关的通路为过氧化物酶体增生物激活受体(PPAR)信号通路与脂肪细胞因子信号通路等。根据转录组数据分析血管生成样素蛋白4 (ANGPTL4)、脂肪酸合成酶(FASN)及肉毒碱棕榈酰基转移酶Ⅰ肝脏亚型(CPT1A)基因与脂肪代谢相关。6月龄与2月龄滩羊羔羊背最长肌共筛选出差异表达基因1 418个,其中774个上调基因,644个下调基因。GO富集主要体现在细胞-细胞黏附、单个生物体的细胞-细胞黏附及单个生物体的细胞黏附,KEGG显著富集在18个通路中,与脂肪代谢密切相关的为PPAR信号通路与脂肪酸代谢等通路。根据转录组数据分析瘦素(LEP)、胰岛素样生长因子2 (IGF2)、心脏型脂肪酸结合蛋白3 (FABP3)基因与脂肪代谢相关。研究表明,舍饲条件下,ANGPTL4、FASN、CPT1A基因在不同月龄滩羊羔羊股二头肌脂肪代谢过程中发生调节作用,而LEP、IGF2、FABP3基因对背最长肌脂肪代谢发挥调节作用。The purpose of this study was to explore the differentially expressed genes related to fat metabolism in muscle tissue of Tan lambs at different ages under the house-feeding mode.Twelve Tantan sheep with similar body condition were selected as the study objects,and three Tantan sheep with similar body weight were selected for slaughter at two-month-old and ix-month-old,respectively,and the longissimus dorsi muscle and biceps femoris muscle were taken.Transcriptome sequencing of the longissimus dorsi muscle was performed by Illumina to screen for genes related to fat metabolism.The results showed that a total of 146 differentially expressed genes were screened from the biceps femoris muscle of six-month-old and two-month-old Tan lambs,of which 69 genes were up-regulated and 77 genes were downregulated.The differentially expressed genes were enriched in 40 GO entries,mainly in biological processes such as the regulation of endothelial apoptosis,carboxylic acid metabolism and epithelial apoptosis.KEGG was significantly enriched in four pathways,and the pathways closely related to fat metabolism were PPAR signaling pathway and adipocytokine signaling pathway.According to transcriptome analysis,ANGPTL4,FASN,and CPT1A genes were associated with fat metabolism.A total of 1 418 differentially expressed genes were screened in the longissimus dorsi muscle of six-month-old and two-month-old Tan lambs,of which 774 genes were up-regulated and 644 genes were downregulated.GO enrichment was mainly reflected in cellcell adhesion,cell-cell adhesion of a single organism and cell adhesion of a single organism,and KEGG was significantly enriched in 18 pathways,among which PPAR signaling pathway and fatty acid metabolism pathway were closely related to fat metabolism.LEP,IGF2,and FABP3 genes were associated with fat metabolism according to transcriptome data.The results showed that ANGPTL4,FASN,and CPT1A genes regulated the fat metabolism of biceps femoris muscle in lambs at different months of age,while the LEP,IGF2,and FABP3 genes

关 键 词：滩羊 舍饲 肌肉组织 脂肪代谢 

分 类 号：S826[农业科学—畜牧学]