小球藻兼养培养基的研究及品质分析  

Research and quality analysis of Chlorella vulgaris mixotrophy medium

作　　者：兰彪 赵羚汐 LAN Biao;ZHAO Ling-xi

机构地区：[1]珠海光藻生命科学有限公司,广东珠海519080 [2]白城师范学院化学学院,吉林白城137000

出　　处：《饲料研究》2024年第5期76-81,共6页Feed Research

基　　金：吉林省重点科技研发项目(项目编号:20180201061NY)。

摘　　要：试验旨在通过优化小球藻兼养培养基,结合工业化生产提高小球藻的生物量及应用潜力。试验以小球藻为试验对象,在L-丙氨酸、酵母水解液、乙酸钠和氰钴胺的单因素试验基础上,以生物量为响应值,利用响应面试验优化兼养培养基的配比组成。结果显示,小球藻兼养培养基最佳配比为L-丙氨酸4.23 g/L、酵母水解液2.62 mL/L、乙酸钠1.44 g/L和氰钴胺0.068μg/L,在此条件下小球藻生物量OD_(680 nm)值达到4.651。在300 L工业化生产验证中,优化培养基生物量是基础培养基的1.8倍,表明优化培养基可以进行工业化实践。试验所得藻粉细腻,色泽墨绿,具有芳草清香,蛋白质含量57.33 g/100 g,水分含量4.40 g/100 g,灰分含量4.87 g/100 g,叶绿素含量40.47 mg/g,铅、砷含量小于0.1 mg/kg,汞、镉未检出;微生物指标均符合行业标准。研究表明,试验结果可为小球藻兼养培养基的工业化开发提供参考依据。The purpose of this experiment was to improve the biomass and application potential of Chlorella vulgaris by optimizing the Chlorella vulgaris mixotrophy medium and combining it with industrial production.In this study,Chlorella vulgaris was used as the test object,and on the basis of the single factor of L-alanine,yeast hydrolysate,sodium acetate,and cyanocobalamin,the biomass was used as the response value,and the ratio of mixotrophy medium was optimized by response surface test.The results showed that the optimal ratio of Chlorella vulgaris mixotrophy medium was L-alanine 4.23 g/L,yeast hydrolysate 2.62 mL/L,sodium acetate 1.44 g/L,and cyanocobalamin 0.068 μg/L,and the OD_(680 nm) values Chlorella vulgaris reached 4.651 under these conditions.In the 300 L industrial production verification,the biomass of the optimized medium was 1.8 times that of the basal medium,indicating that the optimized medium could be used for industrial practice.The obtained algae powder was delicate,dark green,fragrant with herb,the protein content was 57.33 g/100 g,the moisture content was 4.40 g/100 g,the ash content was 4.87 g/100 g,the chlorophyll content was 40.47 mg/g,the lead and arsenic content was less than 0.1 mg/kg,and the mercury and cadmium were not detected.The microbial indicators were in line with the industry standards.The results can provide a reference basis for the industrial development of Chlorella vulgaris mixotrophy medium.

关 键 词：小球藻 L-丙氨酸 兼养培养 品质分析 

分 类 号：S816[农业科学—饲料科学]