分子印迹技术在大环内酯类药物检测中的应用  

Application of molecular imprinting technology in detection of macrolides

作　　者：王瑜 刘岑 杨智翔 段二珍 赵银丽[1] 乔汉桢 王金荣[1] WANG Yu;LIU Cen;YANG Zhi-xiang;DUAN Er-zhen;ZHAO Yin-li;QIAO Han-zhen;WANG Jin-rong

机构地区：[1]河南工业大学生物工程学院,河南郑州450001

出　　处：《饲料研究》2024年第5期172-176,共5页Feed Research

基　　金：河南工业大学青年骨干教师培育计划(项目编号:21421208、21421204)。

摘　　要：分子印迹技术是模拟酶-底物、抗原-抗体之间的相互作用,对印迹分子进行专一识别的技术,能够特异性识别模板或类似物,常被用于分析样品的预处理,可对样品进行高效提取和净化。大环内酯类药物是畜牧业中常用抗生素之一,广泛用于畜禽呼吸道、胃肠道感染等疾病的预防与治疗,但不规范使用可能导致其在动物产品及环境中残留,对人类健康产生威胁。文章针对分子印迹技术对大环内酯类药物残留检测的预处理技术研究进展进行综述,重点对分子印迹固相萃取和磁性分子印迹固相萃取进行介绍,并对分子印迹技术在大环内酯类药物残留分析预处理的应用前景进行展望,为优化大环内酯类抗生素检测前处理方法提供参考。Molecular imprinting technology is a technology that simulates the interaction between enzymes,substrates,antigens and antibodies,and specifically identifies imprinted molecules,which can specifically identify templates or analogues,and is often used in the pretreatment of analytical samples for efficient extraction and purification of samples.Macrolides are one of the commonly used antibiotics in animal husbandry,which are widely used in the prevention and treatment of respiratory tract and gastrointestinal infections in livestock and poultry,but non-standard use may lead to residues in the production of animal products and the environment,posing a threat to human health.The paper reviews the research progress of molecular imprinting technology for the detection of macrolide drug residues,focusing on the introduction of molecularly imprinted solid phase extraction and magnetic molecularly imprinted solid phase extraction,and prospects for the application of molecular imprinting technology in the pretreatment of macrolide drug residue analysis,so as to provide reference for optimizing the pretreatment method of macrolide antibiotics detection.

关 键 词：分子印迹技术 大环内酯类抗生素 分子印迹固相萃取 磁性分子印迹固相萃取 

分 类 号：S816.17[农业科学—饲料科学]