眶上锁孔入路手术建立食蟹猴大脑中动脉闭塞模型  

Taking supraorbital keyhole approach operation to build cynomolgus monkey middle cerebral artery occlusion model

作　　者：初晨宇 徐强 饶军华 李岳锋 Chu Chenyu;Xu Qiang;Rao Junhua;Li Yuefeng(Department of Neurosurgery,the 960th Hospital of People's Liberation Army,Ji'nan 250031,China;Department of Neurosurgery,Beijing Puhua International Hospital,Beijing 100050,China;Guangdong Academy of Sciences,Guangzhou 510260,China;Guangdong Landau Biotechnology Co.Ltd,Guangzhou 510555,China)

机构地区：[1]解放军第九六〇医院神经外科,济南250031 [2]北京天坛普华医院神经外科,北京100050 [3]广东省科学院动物研究所,广州510260 [4]广东蓝岛生物技术有限公司,广州510555

出　　处：《中华脑科疾病与康复杂志（电子版）》2024年第1期8-13,共6页Chinese Journal of Brain Diseases and Rehabilitation（Electronic Edition）

摘　　要：目的应用眶上锁孔入路手术方法建立食蟹猴大脑中动脉闭塞(MCAO)模型并评价模型制作效果。方法4只普通级实验猴随机分为2组,手术组3只实验猴经左侧眶上锁孔入路手术开颅,显露并电凝切断左侧大脑中动脉M1段起始部,建立MCAO模型;对照组1只实验猴经左侧眶上锁孔入路手术开颅,仅显露左侧大脑中动脉M1段起始部,不电凝切断,作为阴性对照。分别于术后24 h、3 d、7 d、14 d、28 d对所有实验猴行非人灵长类动物卒中量表(NHPSS)评分,于术后7 d对所有实验猴行头颅MRI轴位T2加权扫描。结果所有实验猴术后无死亡,无明显术后并发症,实验周期内均存活良好。手术组实验猴术后24 h、3 d、7 d、14 d、28 d的NHPSS评分分别为(19.67±1.15)、(22.00±1.00)、(19.33±1.15)、(18.67±1.53)、(18.00±1.00)分。对照组实验猴术后24 h、3 d、7 d、14 d、28 d的NHPSS评分均为0分。头颅MRI轴位T2WI提示:手术组实验猴左侧大脑中动脉支配区域脑组织均出现梗死灶,分布于左侧额叶、顶叶、颞叶及基底节区的部分区域,伴有轻度脑水肿;对照组实验猴脑内无梗死灶及脑水肿形成。结论应用眶上锁孔入路手术方法造模具备微创优势,模型效果可靠,重复性好,适合进行食蟹猴MCAO模型的批量制作。Objective To evaluate the effectiveness of building cynomolgus monkey middle cerebral artery occlusion(MCAO)model by taking supraorbital keyhole approach operation.Methods Four ordinary grade experimental monkeys were randomly divided into two groups.Three experimental monkeys of operation group underwent left supraorbital keyhole approach craniotomy,initial part of M1 segment of left middle cerebral artery was exposed and then coagulated and cut to make MCAO model.One experimental monkey of control group underwent left supraorbital keyhole approach craniotomy,initial part of M1 segment of left middle cerebral artery was exposed but not coagulated and cut to make negative control.All experimental monkeys were evaluated using the non-human primate stroke scale(NHPSS)at 24 h,3 d,7 d,14 d,and 28 d postoperatively.Brain MRI axial T2 weighted scans were performed on all experimental monkeys at 7 d postoperatively.Results All experimental monkeys survived after operation without any significant postoperative complications,and lived well during experimental period.The NHPSS scores of the operation group at postoperative time of 24 h,3 d,7 d,14 d,28 d was(19.67±1.15),(22.00±1.00),(19.33±1.15),(18.67±1.53),(18.00±1.00)points respectively.The NHPSS scores of the control group at postoperative time of 24 h,3 d,7 d,14 d,28 d were all 0 points.Brain MRI axial T2 weighted scan at 7 d after operation showed that brain infarction focus appeared in the blood supply area of left middle cerebral artery of all experimental monkeys of operation group,scattered in the left frontal lobe,parietal lobe,temporal lobe and basal ganglion area,and accompanied with mild cerebral edema,whereas no brain infarction focus and brain edema was appeared in the experimental monkey of control group.Conclusion The application of the supraorbital keyhole approach surgical method has the advantage of minimally invasion,reliable model effect,good repeatability.The approach method has good repeatability and can make mass production of cynomolgus monke

关 键 词：大脑中动脉闭塞 眶上 锁孔入路 食蟹猴 动物模型 

分 类 号：R651.3[医药卫生—外科学]