Isolation and culture of human primary osteoblasts:Comparing the effects of differences in method details on osteoblast characteristics  

作　　者：Fei Chen Yan Zhang Pai Peng Xiao-Ting Huang Zi-Han Qiu Bao-Cheng Liu Tie-Lin Yang Bo Yang Yan Guo 

机构地区：[1]Key Laboratory of Biomedical Information Engineering of Ministry of Education,Biomedical Informatics & Genomics Center,School of Life Science and Technology,Xi'an Jiaotong University,Xi'an,Shaanxi 710049,China [2]Department of Orthopaedics,Shannxi Provincial People's Hospital (The Third Affiliated Hospital of Xi'an Jiaotong University),Xi'an,Shaanxi 710068,China

出　　处：《Genes & Diseases》2024年第2期546-549,共4页基因与疾病（英文）

基　　金：supported by the National Natural Science Foundation of China(No.82170896,31970569);Science Fund for Distinguished Young Scholars of Shaanxi Province,China(No.2021JC-02);Innovation Capability Support Program of Shaanxi Province,China(No.2022TD-44);China Postdoctoral Science Foundation(No.2021M692582);Natural Science Basic Research Program of Shaanxi Province,China(No.2022JQ-808,2023-YBSF-180);Fundamental Research Funds for the Central Universities(China)(No.xzy012022032).

摘　　要：Osteoblasts are essential in the maintenance of human bone homeostasis.1 The abnormal formation and impaired differentiation ability of osteoblasts are pivotal factors leading to bone-related diseases,suggesting that an indepth study of osteoblasts can provide novel treatment strategies for these diseases.2 Human primary osteoblasts are precious experimental models in bone-related research.3 However,the isolation and culture of human primary osteoblasts still have some difficulties and limitations.Here,we refined a method to isolate and culture human primary osteoblasts by taking cancellous bone through a bone tissue sampler and pre-digesting it with pancreatin,which makes the process easier,cheaper,and more efficient.Furthermore,we compared the effects of the subtle changes in isolation and culture methods on the phenotype of human primary osteoblasts and examined the differences in morphology,proliferation,and differentiation between micro-explants cultured osteoblasts(MECOBs)and explants cultured osteoblasts(EC-OBs).

关 键 词：IMPAIRED CULTURE DETAILS 

分 类 号：R329.2[医药卫生—人体解剖和组织胚胎学]