Vitamin A deficiency suppresses CEACAM1 to impair colonic epithelial barrier function via downregulating microbial-derived short-chain fatty acids  

作　　者：Junyan Yan Lu Xiao Di Feng Baolin Chen Ting Yang Bei Tong Ruifang Luo Yuting Wang Jie Chen 

机构地区：[1]Children Nutrition Research Center,Children's Hospital of Chongqing Medical University,Chongqing Key Laboratory of Child Nutrition and Health,Ministry of Education Key Laboratory of Child Development and Disorders,National Clinical Research Center for Child Health and Disorders,Chongqing 401122,China [2]Department of Gastroenterology,Children's Hospital of Chongqing Medical University,Chongqing 401122,China

出　　处：《Genes & Diseases》2024年第2期1066-1081,共16页基因与疾病（英文）

基　　金：supported by grants from The National Natural Science Foundation of China(No.31971089);the General Project of Chongqing Natural Science Foundation(China)(No.CSTB2022NSCQ-MSX0107);the Scientific Research Innovation Project for Postgraduates in Chongqing City(China)(No.CYS19198).

摘　　要：Vitamin A (VA) plays an essential role in modulating both the gut microbiota and gut barrier function.Short-chain fatty acids (SCFAs),as metabolites of the gut microbiota,protect the physiological intestinal barrier;however,they are compromised when VA is deficient.Thus,there is an urgent need to understand how and which SCFAs modulate colonic epithelial barrier integrity in VA deficiency (VAD).Herein,compared with normal VA rats (VAN),at the beginning of pregnancy,we confirmed that the colonic desmosome junction was impaired in the VAD group,and the amounts of acetate,propionate,and butyrate declined because of the decreased abundance of SCFA-producing bacteria (Romboutsia ,Collinsella ,and Allobaculum ).The differentially expressed genes correlated with the gut barrier and the histone deacetylase complex between the VAD and VAN groups were enriched by RNA sequencing.In the VAD group,the expression levels of colonic CEA cell adhesion molecule 1 (CEACAM1) were down-regulated,and the levels of histone deacetylase 1 (HDAC1) and HDAC3 were up-regulated.Intriguingly,the above changes in the VAD groups were rescued by VA supplementation in the early postnatal period.Further study indicated that in Caco-2 cells,butyrate treatment significantly repressed the enrichment of HDAC3 on the promoter of the CEACAM1 gene to induce its expression.Our findings support that butyrate intervention can alleviate the impairment of colonic barrier function caused by VAD,and timely postnatal VA intervention may reverse the damage caused by VAD on gut barrier integrity during pregnancy.

关 键 词：CEACAM1 Colonic epithelial barrier HDAC3 Short-chain fatty acids Vitamin A deficiency 

分 类 号：R725.7[医药卫生—儿科]