Identification of carbohydrate in Polygonatum sibiricum:fructo-oligosaccharide was a major component  

作　　者：Jiabei Xia Cenrong Zhang Kai Zhu Xingyu Mei Huan Cheng Shiguo Chen Xingqian Ye Jianle Chen 

机构地区：[1]Ningbo Innovation Center,Zhejiang University,Ningbo,China [2]College of Biosystems Engineering and Food Science,National–Local Joint Engineering Laboratory of Intelligent Food Technology and Equipment,Zhejiang Key Laboratory for Agri-Food Processing,Fuli Institute of Food Science,Zhejiang Engineering Laboratory of Food Technology and Equipment,Zhejiang University,Hangzhou,China [3]Zhejiang University Zhongyuan Institute,Zhengzhou,China [4]School of Biological and Chemical Engineering,NingboTech University,Ningbo,China

出　　处：《Food Quality and Safety》2023年第3期439-446,共8页食品品质与安全研究（英文版）

基　　金：This work was financially supported by the grants from Ningbo Municipal Bureau of Science and Technology(2022Z174);The Major Agricultural Technology Collaborative Promotion Plan of Zhejiang Province(2022XTTGZYC03);The Key Research and Development Program of Zhejiang Province(2021C02001),China.

摘　　要：Polygonatum sibiricum,famous for its physiological activities,is a popular Chinese food and a traditional Chinese herb containing many carbohydrates as the main component.However,except for most reported polysaccharides,other detailed compositions of carbohydrates are still not clear.To verify the controversial existence of starch and investigate other components,especially oligosaccharides,we used iodine–potassium iodide colorimetric detection and enzymatic hydrolysis to determine starch.Then,oligosaccharides were analyzed by thin-layer chromatography,gel permeation chromatography,high-performance anion exchange chromatography with pulsed amperometric detection,and hydrophilic interaction chromatography–electrospray tandem mass spectrometry.The results showed that the rhizome of P.sibiricum lacked starch,and fructooligosaccharides were the main component,accounting for approximately 28.95%.Oligosaccharides with degrees of polymerization above 10 were the most abundant components.This study clearly illustrated the unknown carbohydrate components of the Polygonatum rhizome,promoting its functional value with new evidence.

关 键 词：Polygonatum sibiricum starch determination FRUCTO-OLIGOSACCHARIDES HPAEC-PAD HILIC-ESI-MS/MS 

分 类 号：TS201.23[轻工技术与工程—食品科学]