Comparative analysis of fruit firmness and genes associated with cell wall metabolisms in three cultivated strawberries during ripening and postharvest  

作　　者：任奕博 李柏君 贾浩然 杨肖芳 孙云帆 寿佳涵 蒋桂华 石艳娜 陈昆松 Yibo Ren;Baijun Li;Haoran Jia;Xiaofang Yang;Yunfan Sun;Jiahan Shou;Guihua Jiang;Yanna Shi;Kunsong Chen(College of Agriculture and Biotechnology,Zhejiang University,Zijingang Campus,Hangzhou,China;Zhejiang Provincial Key Laboratory of Horticultural Plant Integrative Biology,Zhejiang University,Zijingang Campus,Hangzhou,China;State Agriculture Ministry Laboratory of Horticultural Plant Growth,Development and Quality Improvement,Zhejiang University,Zijingang Campus,Hangzhou,China;Institute of Horticulture,Zhejiang Academy of Agricultural Sciences,Hangzhou,China)

机构地区：[1]College of Agriculture and Biotechnology,Zhejiang University,Zijingang Campus,Hangzhou,China [2]Zhejiang Provincial Key Laboratory of Horticultural Plant Integrative Biology,Zhejiang University,Zijingang Campus,Hangzhou,China [3]State Agriculture Ministry Laboratory of Horticultural Plant Growth,Development and Quality Improvement,Zhejiang University,Zijingang Campus,Hangzhou,China [4]Institute of Horticulture,Zhejiang Academy of Agricultural Sciences,Hangzhou,China

出　　处：《Food Quality and Safety》2023年第3期516-524,共9页食品品质与安全研究（英文版）

基　　金：supported by the Key Research and Development Program of Shandong Province,China(No.2022TZXD0022);the National Natural Science Foundation of China(Nos.32102345 and 32002004);the 111 Project(No.B17039),China.

摘　　要：Cultivated strawberry(Fragaria×ananassa),a world-famous fruit,is subjected to rapid softening during ripening,resulting in a shorter shelf life and severe economic losses during storage and transportation.However,there is limited understanding of the molecular mechanism underlying differences in fruit firmness during ripening and postharvest among cultivated strawberries.Here,we explored this molecular mechanism by comparing three cultivated strawberries via firmness measurement,transcriptome analysis,quantitative real-time polymerase chain reaction,and correlation analysis,and revealed FaEXP7,FaPG2,FaPLA,and Faβ-Gal4 as potential softening activators expressed before harvest to determine fruit with more softened texture and shorter shelf life,and that extremely high expression levels of FaCEL1-1 and FaCEL1-3 during ripening might be accelerators to intensify this situation.Additionally,both the enzyme activities of FaCEL and the expression pattern of FaCEL1-3 showed a significantly negative correlation with fruit firmness after harvest,suggesting that FaCEL1-3 might play a key role in promoting strawberry fruit softening not only during ripening but also postharvest.These results showed that the difference in fruit firmness and shelf life among cultivated strawberries was controlled by the temporal expression pattern of a legion of cell wall-associated genes during ripening and postharvest.

关 键 词：Fragaria×ananassa fruit cell wall softening RIPENING POSTHARVEST 

分 类 号：S668.4[农业科学—果树学]