Optimization of nucleic acid extraction methods for rapid detection in pandemic situations or diseases with high prevalence  

作　　者：Hesti Lina Wiraswati Ilma Fauziah Ma'ruf Savira Ekawardhani Lia Faridah Amila Laelalugina Harry Septanto Imam Damar Djati Shabarni Gaffar Asif Awaludin 

机构地区：[1]Department of Biomedical Sciences,Faculty of Medicine Universitas Padjadjaran,Bandung,Indonesia [2]Oncology and Stem Cell Working Group,Universitas Padjadjaran,Bandung,Indonesia [3]Research Center for Climate and Atmosphere,National Research and Innovation Agency,Jl.Sangkuriang,Dago,Bandung,40135,Indonesia [4]Research Center for Smart Mechatronics,National Research and Innovation Agency(BRIN),Bandung,Indonesia [5]Bandung Institute of Technology,Faculty of Visual Art and Design,Jalan Ganesha 10,Bandung,West Java,Indonesia [6]Department of Chemistry,Faculty of Mathematics and Natural Sciences,Universitas Padjadjaran,Sumedang,Indonesia

出　　处：《Journal of Pharmaceutical Analysis》2023年第12期1577-1579,共3页药物分析学报（英文版）

基　　金：supported by the Research and Innovation for Advanced Indonesia(RIIM)Funding Program of the Republic of Indonesia's National Research and Innovation Agency(BRIN)(Program No.44/IV/KS/06/2022).

摘　　要：Nucleic acid extraction(NAE)is an essential component of many assays used for diagnostic and research purposes.During a pandemic,performing NAE is associated with significant challenges due to material shortages,high costs,limited laboratory facilities,and the time required.This delays patient diagnosis and treatment,particularly in countries that do not produce NAE devices.Here,we tested several procedures to speed up the extraction process using common laboratory reagents,finding that heat-based nucleic acid extraction of severe acute respiratory syndrome coronavirus 2(SARS-COV2)is a detection method that can be easily implemented in laboratories,even those without centrifugation facilities.

关 键 词：DIAGNOSIS HIGH finding 

分 类 号：R446[医药卫生—诊断学]