改良大鼠挫伤型脊髓损伤模型的制备与评估  

Preparation and evaluation of an improved model of contusion spinal cord injury in rats

作　　者：陈业煌 陈恺钦 薛亮 吴箭午 黄预备 魏梁锋 曾炳香 王守森 Chen Yehuang;Chen Kaiqin;Xue Liang;Wu Jianwu;Huang Yubei;Wei Liangfeng;Zeng Bingxiang;Wang Shousen(Department of Neurosurgery,Fuzong Clinical Medical College of Fujian Medical University(The 900th Hospital of Joint Logistic Team of PLA),Fuzhou 350025,China)

机构地区：[1]福建医科大学福总临床医学院(联勤保障部队第九〇〇医院)神经外科,福州350025

出　　处：《中华神经创伤外科电子杂志》2023年第6期325-332,共8页Chinese Journal Of Neurotraumatic Surgery：Electronic Edition

基　　金：福建省自然科学基金(2021J011276);联勤保障部队第九OO医院临床应用研究专项(2020L18);联勤医学重点专科项目(LQZD-SW)。

摘　　要：目的利用新型脊髓损伤(SCI)打击器制备大鼠挫伤型SCI模型,并对模型进行评估,为探究SCI后神经病理性疼痛(NP)发生机制及早期干预治疗奠定基础。方法100只健康成年雄性SD大鼠按随机数字表法分为Control组(对照组,20只)、Sham组(假手术组,40只)及SCI组(40只)。SCI组大鼠暴露T10节段并采用改进型Allen's法制备SCI模型,假手术组只暴露T10节段但不实施脊髓撞击,Control组不做任何干预措施。使用开放场地实验(BBB评分)评估伤前1 d及伤后1、3、7、14、21、28 d大鼠的运动功能。于伤前1 d及伤后14、21、28 d测定后肢机械缩足阈值(MWT),并采用苏木精-伊红(HE)染色检测脊髓组织病理学变化,评估SCI模型是否构建成功。结果(1)3组大鼠伤后1、3、7、14、21、28 d的BBB评分比较,差异均有统计学意义(P<0.05),其中SCI组的BBB评分均低于Control组和Sham组,差异有统计学意义(P<0.05)。SCI组伤后1 d的BBB评分均为0分,伤后3、7、14、21、28 d呈上升趋势,且任意两个时间点比较差异均有统计学意义(P<0.05)。(2)3组大鼠伤前1 d后肢MWT比较差异无统计学意义(P>0.05),伤后14、21、28 d后肢MWT比较,差异有统计学意义(P<0.05),且SCI组大鼠后肢MWT均明显低于Control组和Sham组,差异有统计学意义(P<0.05)。SCI组大鼠伤前1 d及伤后14、21、28 d时的后肢MWT呈现先下降后回升趋势,伤后21和28 d比较差异无统计学意义(P>0.05),其余各时间点后肢MWT比较差异均有统计学意义(P<0.05)。(3)伤后14 d,HE染色可见脊髓胶质细胞大量浸润;伤后21 d胶质细胞大量分化增生;伤后28 d大量胶质纤维生成。结论利用新型打击器建立的挫伤型SCI模型,具有数字化、稳定、标准化、操作性强、重复性好的特点,可为SCI后NP形成的病理学机制及靶向药物研究提供标准化的模型。Objective To prepare a rat contusion SCI model using a novel spinal cord injury(SCI)impactor and evaluate the model,in order to lay the foundation for exploring the mechanism of neuropathic pain after SCI and early intervention and treatment.Methods One hundred adult male SD rats were randomly divided into Control group(n=20),Sham group(n=40)and SCI group(n=40).Rats in SCI group were exposed to T10 segments and the SCI model was established by modified Allen's method.Rats in Sham group were exposed to T10 segments without SCI,while rats in Control group were not intervened.Open field test(BBB score)was used to evaluate the motor function of rats 1 d before injury and 1,3,7,14,21 and 28 d after injury.The mechanical foot retraction threshold(MWT)of the hind limb was measured 1 d before injury and 14,21 and 28 d after injury,and the histopathological changes of the spinal cord were detected by hematoxylin eosin(HE)staining to evaluate whether the SCI model was successfully constructed.Results(1)The BBB scores of three groups of rats at 1,3,7,14,21,and 28 d after injury were compared,and the differences were statistically significant(P<0.05).Among them,the BBB scores of the SCI group were lower than those of the Control group and the Sham group,and the differences were statistically significant(P<0.05).The BBB scores of the SCI group were all 0 points on the 1st day after injury,and showed an upward trend at 3,7,14,21,and 28 d after injury,and the difference between any two time points in pairwise comparison was statistically significant(P<0.05).(2)There was no statistically significant difference in rat hindlimb MWT among the three groups 1 d before injury(P>0.05),but there was a statistically significant difference at 14,21,and 28 d after injury(P<0.05).Moreover,the rat hindlimb MWT of SCI group was significantly lower than that of the Control group and Sham group,and the difference was statistically significant(P<0.05).The rat hindlimb MWT of SCI group rats showed a trend of first decreasing and then rebounding a

关 键 词：挫伤型脊髓损伤 改良动物模型 神经病理性疼痛 机械缩足阈值 

分 类 号：R651.2[医药卫生—外科学]