姜黄素调控Yes相关蛋白1对子宫内膜癌Warburg效应及生物学行为的影响  

Effects of Curcumin Regulating Yes-Associated Protein 1 on Warburg Effect and Biological Behaviors of Patientss with Endometrial Carcinoma

作　　者：胡颜霞[1] 宋晓霞[1] 张喜红[1] HU Yanxia;SONG Xiaoxia;ZHANG Xihong(Department of Gynecology,People's Hospital of Zhengzhou,Zhengzhou Henan 450052,China)

机构地区：[1]郑州人民医院妇科,河南郑州450052

出　　处：《新中医》2024年第5期207-215,共9页New Chinese Medicine

基　　金：河南省重点科技攻关项目(162102310233)。

摘　　要：目的:观察姜黄素调控Yes相关蛋白1 (YAP1)对子宫内膜癌糖酵解Warburg效应及生物学行为的影响。方法:将HEC-1B细胞分为对照组、NC-YAP1组、si-YAP1组及低、中、高剂量组。对照组不做任何处理,NC-YAP1组转染NC-YAP1质粒,si-YAP1组转染si-YAP1质粒,低、中、高剂量组细胞分别用20、40、80μmol/L的姜黄素处理。试剂盒检测各组细胞葡萄糖消耗量及乳酸含量;CCK-8法检测各组细胞增殖能力;流式细胞术检测各组细胞周期与凋亡;划痕法检测各组细胞迁移能力;Transwell法检测各组细胞侵袭能力。蛋白质印迹(Western blot)法检测YAP1、乳酸脱氢酶-A (LDH-A)、己糖激酶2 (HK2)、丙酮酸激酶2 (PKM2)蛋白表达。结果:与对照组比较,NC-YAP1组YAP1蛋白相对表达水平、乳酸含量及葡萄糖消耗量、LDH-A、HK2、PKM2蛋白水平、细胞增殖抑制率、G0/G1期比例及S期比例、细胞凋亡率、划痕愈合率、细胞侵袭抑制率差异均无统计学意义(P>0.05),si-YAP1组及低、中、高剂量组YAP1蛋白相对表达水平、葡萄糖消耗量及乳酸含量、LDH-A、HK2、PKM2蛋白水平、S期比例、划痕愈合率均降低(P<0.05),细胞增殖抑制率、G0/G1期比例、细胞凋亡率、细胞侵袭抑制率均升高(P<0.05)。与NC-YAP1组比较,siYAP1组及低、中、高剂量组细胞YAP1蛋白相对表达水平、葡萄糖消耗量及乳酸含量、LDH-A、HK2、PKM2蛋白水平、S期比例、划痕愈合率均降低(P<0.05),细胞增殖抑制率、G0/G1期比例、细胞凋亡率细胞侵袭抑制率均升高(P<0.05),且低、中、高剂量组上述指标均呈剂量依赖性(P<0.05)。与si-YAP1组比较,低、中剂量组细胞中YAP1蛋白相对表达水平均增加(P<0.05),而高剂量组差异无统计学意义(P>0.05);低剂量组葡萄糖消耗量及乳酸分泌量、LDH-A、HK2、PKM2蛋白水平、S期比例、划痕愈合率均增加(P<0.05),细胞增殖抑制率、G0/G1期比例、细胞凋亡率、细胞侵袭抑制率均降低Objective:To observe the effects of curcumin regulating Yes-associated protein 1(YAP1)on the Warburg effect of endometrial carcinoma glycolysis and on biological behaviors.Methods:The HEC-1B cells were divided into the control group,the NC-YAP1 group,the si-YAP1 group,and the low-dose,medium-dose and high-dose of curcumin groups.The control group was not given any treatment:the NC-YAP1 group was transfected with NC-YAP1 plasmid;the si-YAP1 group Was transfected with si-YAP1 plasmid,and the low-dose,medium-dose and high-dose of curcumin groups were respectively treated with 20,40 and 80μmol/L curcumin.The consumption of glucose by cells and the lactic acid content in each group were detected by kits;the reproductive capacity of cells in each group was detected by the CCK-8 method;the cell cycle and apoptosis in each group were detected by flow cytometry;the cell migration ability in each group was detected by scratch test,and the cell invasion ability in each group was detected by Transwell method.The protein expressions of YAP1,lactate dehydrogenase-A(LDH-A),hexokinase 2(HK2)and pyruvate kinase M2(PKM2)were detected by the Western blot method.Results:There Was no significance being found in the comparisons of protein expression levels of YAP1,lactic acid content,consumption of glucose,protein levels of LDH-A,HK2 and PKM2,activity and inhibition rates of cell proliferation,ratios of Go/G1 phase and S phase,apoptosis rates,scratch healing rates,and inhibition rates of cell invasion between the NC-YAP1 group and the control group(P>0.05).Compared with those in the control group,the protein expression levels of YAP1,consumption of glucose,lactic acid content,protein levels of LDH-A,HK2 and PKM2,activity of cell proliferation,ratios of S phase and scratch healing rates in the si-YAP1 group and the low-dose,mediumdose and high-dose of curcumin groups were decreased(P<0.05),and the inhibition rates of cell proliferation,ratios of Go/G1 phase,apoptosis rates and inhibition rates of cellinvasion were increased(P<0.05).Co

关 键 词：子宫内膜癌 姜黄素 Yes相关蛋白1 Warburg效应 

分 类 号：R285.5[医药卫生—中药学]