BOD5荧光测定法与标准方法在地表水监测中的对比性研究  

Comparative Study of BOD5 Fluorescence Method and Standard Method in Natural Water Monitoring

作　　者：张卫宏 武宇芳[2] 曹轶男[3] 于哲 董建民 ZHANG Weihong;WU Yufang;CAO Yinan;YU Zhe;DONG Jianmin(CECEP Talroad Technology Co.Ltd.,Beijing 100089,China;Xinzhou Normal Unversity,Xinzhou 034000,Shanxi China;Liaoning Provincial Institute of Safety Sciences,Shenyang 110004,China)

机构地区：[1]中节能天融科技有限公司,北京100089 [2]山西省忻州师范学院,山西忻州034000 [3]辽宁省安全科学研究院,辽宁沈阳110004

出　　处：《分析测试技术与仪器》2024年第2期110-117,共8页Analysis and Testing Technology and Instruments

基　　金：中国节能环保集团公司重大科技创新项目(项目编号:cecep-zdkj-2020-007),基于微流控芯片技术的水环境微生物快速分析方法研究和开发-水质五日生化需氧量(BOD5)。

摘　　要：BOD5检测是一种基于微生物的污染负荷评估,有文献证明微生物代谢物类色氨酸的荧光强度与BOD5测量之间存在相关性.对长江流域常州段地表水水样的荧光强度与BOD5值之间的关系进行了探究,并通过考察浊度、pH、离子干扰和区域差异来评估荧光法应用于水质BOD5污染评价中的潜力.研究发现,地表水的pH和离子干扰对荧光法检测BOD5并不构成明显影响.区域差异对比结果t最大值为1.227,不构成显著性差异.荧光法水样检测结果与标准方法相比,相关性系数r为0.9099,具有明显相关性.结果表明,荧光法应用于地表水BOD5检测影响因素少、检测速度快、自动化程度高、结果稳定可靠,可以作为BOD5检测的替代方法,成为表征水中可生物降解污染物污染程度的主要手段.BOD5 detection is a microbial-based pollution load assessment,there are many articles demonstrating that correlation between the fluorescence intensity of microbial metabolite(tryptophan-like)and BOD5 measurement.The relationship between the fluorescence intensity and BOD5 value of surface water samples in Changzhou section of the Yangtze River Basin was compared and verified.Turbidity,pH,electrical conductivity and regional differences were investigated to evaluate the potential of fluorescence method for BOD5 pollution assessment.It was found that the pH and ion interference of surface water had no significant effect on the detection of BOD5 by fluorescence method.The maximum value of t was 1.227,which no significant effect.Compared with the standard method,the correlation coefficient of fluorescence method r was 0.9099,which had obvious correlation.The results showed that the fluorescence method has few influencing factors,fast detection speed,high degree of automation,and stable and reliable results when applied to BOD5 detection in surface water, and can be used as an alternative method for BOD5 detection and the main means to characterize the pollution degree of biodegradable pollutants in water.

关 键 词：五日生化需氧量 类色氨酸 荧光法 快速检测 

分 类 号：O657.39[理学—分析化学]