系统性红斑狼疮患者外周血中γδT细胞及其主要功能亚群的变化及临床意义  

Changes and clinical significance ofγδT cells and their major functional subsets in peripheral blood of patients with systemic lupus erythematosus

作　　者：颜云霞 张卓亚[1] 黄赛赛[1] 耿林玉[1] YAN Yunxia;ZHANG Zhuoya;HUANG Saisai;GENG Linyu(Department of Rheumatology and Immunology,Drum Tower Hospital Affiliated to Medical College of Nanjing University,Nanjing,Jiangsu,210008)

机构地区：[1]南京大学医学院附属鼓楼医院风湿免疫科,江苏南京210008

出　　处：《实用临床医药杂志》2024年第6期88-93,共6页Journal of Clinical Medicine in Practice

基　　金：江苏省南京市杰出青年基金项目(JQX20004);南京鼓楼医院临床研究培育项目(2021-LCYJ-PY-16)。

摘　　要：目的探讨系统性红斑狼疮(SLE)患者外周血中γδT细胞及其主要功能亚群γδT1、γδT17细胞的变化及其临床意义。方法采用流式细胞术检测21例SLE患者(SLE组)、16例健康对照患者(HC组)外周血γδT细胞、γδT1细胞、γδT17细胞的比例及绝对数。采用Pearson相关性分析法分析外周血γδT细胞、γδT1细胞、γδT17细胞与临床指标的相关性。结果SLE患者外周血γδT细胞占淋巴细胞百分率为(2.30±1.10)%,低于HC组的(3.30±1.51)%,差异有统计学意义(P<0.05);γδT1细胞占淋巴细胞百分率为(0.93±0.67)%,低于HC组的(2.09±1.21)%,差异有统计学意义(P<0.001);γδT17细胞占淋巴细胞比率为(1.53±2.82)‰,与HC组的(0.18±0.12)‰比较有增多的趋势,但差异无统计学意义(P>0.05)。SLE患者外周血γδT细胞绝对数为(2.88±2.18)×10^(4)/mL,低于HC组的(7.96±3.96)×10^(4)/mL,差异有统计学意义(P<0.0001);SLE患者外周血γδT1细胞绝对数为(1.20±1.17)×10^(4)/mL,低于HC组的(5.05±3.04)×10^(4)/mL,差异有统计学意义(P<0.0001);SLE患者外周血γδT17细胞绝对数为(1.03±1.08)×103/mL,高于HC组的(0.40±0.24)×103/mL,差异有统计学意义(P<0.05)。SLE患者外周血中γδT17细胞比例与系统性红斑狼疮疾病活动度评分(SLEDAI)(r=0.59,P<0.01)、红细胞沉降率(r=0.65,P<0.01)呈正相关,γδT17细胞绝对数与SLEDAI(r=0.59,P<0.01)呈正相关,与补体C3水平(r=-0.53,P<0.05)呈负相关。结论SLE患者外周血中γδT细胞数量显著下降主要由其γδT1细胞亚群减少所致,γδT17细胞在SLE发病及活动中发挥了重要作用。Objective To investigate the changes and clinical significance ofγδT cells and their major functional subsetsγδT1 andγδT17 cells in the peripheral blood of patients with systemic lupus erythematosus(SLE).Methods Ratios and absolute numbers ofγδT cells,γδT1 cells andγδT17 cells in peripheral blood were detected by flow cytometry in 21 SLE patients(SLE)group and 16 healthy controls(HC group).Correlations ofγδT cells,γδT1 cells andγδT17 cells in the peripheral blood with clinical indicators were analyzed by Pearson correlation analysis.Results In SLE patients,the percentage ofγδT cells to lymphocytes in the peripheral blood was(2.30±1.10)%,which was significantly lower than(3.30±1.51)%in the HC group(P<0.05);the percentage ofγδT1 cells to lymphocytes in peripheral blood was(0.93±0.67)%,which was significantly lower than(2.09±1.21)%in the HC group(P<0.001);the ratio ofγδT17 cells to lymphocytes in the peripheral blood was(1.53±2.82)‰,which showed an increasing trend when compared to(0.18±0.12)‰in the HC group(P>0.05).The absolute number ofγδT cells in the peripheral blood in SLE patients was(2.88±2.18)×10^(4)/mL,which was significantly lower than(7.96±3.96)×10^(4)/mL in the HC group(P<0.0001);the absolute number ofγδT1 cells in the peripheral blood in SLE patients was(1.20±1.17)×10^(4)/mL,which was significantly lower than(5.05±3.04)×10^(4)/mL in the HC group(P<0.0001);the absolute number ofγδT17 cells in the peripheral blood in SLE patients was(1.03±1.08)×103/mL,which was significantly higher than(0.40±0.24)×103/mL in the HC group(P<0.05).The proportion ofγδT17 cells in the peripheral blood of SLE patients was positively correlated with the Systemic Lupus Erythematosus Disease Activity Index(SLEDAI)(r=0.59,P<0.01)and erythrocyte sedimentation rate(r=0.65,P<0.01),while the absolute number ofγδT17 cells was positively correlated with the SLEDAI score(r=0.59,P<0.01)and negatively correlated with the complement C3 level(r=-0.53,P<0.05).Conclusion The significant de

关 键 词：系统性红斑狼疮 ΓΔT细胞 干扰素-Γ 白细胞介素-17A 系统性红斑狼疮疾病活动度评分 

分 类 号：R593.24[医药卫生—内科学] R392.7[医药卫生—临床医学] R319