氮掺杂碳量子点的制备及在细胞温度传感中的应用  

Preparation and Application of Nitrogen-doped Carbon Quantum Dots in Cell Temperature Sensing

作　　者：陈雨昕 胡月芳[1] CHEN Yu-xin;HU Yue-fang(Guangxi Key Laboratory of Calcium Carbonate Resources Comprehensive Utilization,College of Materials and Chemical Engineering,Hezhou University,Hezhou 542899,China;Faculty of Chinese Medicine Science,Guangxi University of Chinese Medicine,Nanning 530000,China)

机构地区：[1]贺州学院广西碳酸钙资源综合利用重点实验室材料与化学工程学院,广西贺州542899 [2]广西中医药大学赛恩斯新医药学院,广西南宁530000

出　　处：《分析测试学报》2024年第4期566-573,共8页Journal of Instrumental Analysis

基　　金：广西自然科学基金面上项目(2022GXNSFAA035469,2017GXNSFAA198274);贺州学院博士和教授科研启动基金项目(HZUBS202105,HZUJS202204);广西科技计划项目(桂科AD23023008)。

摘　　要：该研究以生物质西红柿为碳源和氮源,通过微波法制备了氮自掺杂碳量子点(N-CQDs)。通过场发射透射电子显微镜(TEM)、X射线光电子能谱仪(XPS)和荧光光度计等对其形貌、结构、稳定性、光电性能、温敏性及细胞毒性进行考察,并用于活细胞温度传感研究。实验结果表明,所制备的N-CQDs稳定性强,光电性能优异,对温度具有较强的敏感性。在25~65℃范围内,N-CQDs的荧光强度随着温度的升高而降低,两者呈良好的线性关系。N-CQDs能穿透4T1细胞的细胞膜,显示出低毒性和生物相容性,在4T1细胞中具有良好的温度传感性能。该N-CQDs在活细胞荧光温度传感领域具有较大的应用潜能,有望作为温敏型纳米荧光探针在细胞研究等生物医学领域得到广泛应用。In this study,nitrogen self-doped carbon quantum dots(N-CQDs)were prepared by mi⁃crowave method using biomass tomato as carbon and nitrogen sources.The morphology,structure,stability,photoelectric properties,temperature sensitivity and cytotoxicity were studied by field emission transmission electron microscope(TEM),X-ray photoelectron spectrometer(XPS)and fluo⁃rescence luminescence photometer,and were applied to temperature sensing in living cells.The ex⁃perimental results show that the prepared N-CQDs has strong stability,excellent photoelectric perfor⁃mance and strong sensitivity to temperature.In the temperature range of 25-65℃,the fluorescence intensity of N-CQDs decreases with the increase of temperature,and shows a good linear relation⁃ship.The N-CQDs can penetrate the cell membrane of 4T1 cells,showing low toxicity and biocom⁃patibility,and good temperature sensing fluorescence properties in 4T1 cells.It is indicated that the N-CQDs has great potential and value in the field of fluorescence temperature sensing in living cells,and is of great significance in the development of non-destructive cell measurement of nanoferters.It is expected to become a temperature-sensitive fluorescence nanoprobe and be widely used in biomedi⁃cal fields such as cell research.

关 键 词：氮自掺杂碳量子点 微波法 光电性能 温敏性能 细胞温度传感 

分 类 号：O657.3[理学—分析化学] TB332[理学—化学]