解酒护肝胶囊对大鼠酒精性肝损伤保护作用研究  

Protection effect of alcoholic liver injury by Jiejiu Hugan Capsules in rats

作　　者：庞宇辰 郑俊侨 张思璐 李海龙 吴欣颖 肖国勋 时军 PANG Yuchen;ZHENG Junqiao;ZHANG Silu;LI Hailong;WU Xinying;XIAO Guoxun;SHI Jun(Shenzhen Baoshijian Biotechnology Co.Ltd.,Shenzhen 518116,China;School of Chinese Materia Medica,Guangdong Pharmaceutical University,Guangzhou 510006,China)

机构地区：[1]深圳保时健生物工程有限公司,广东深圳518116 [2]广东药科大学中药学院,广东广州510006

出　　处：《广东药科大学学报》2024年第2期1-6,共6页Journal of Guangdong Pharmaceutical University

摘　　要：目的评价解酒护肝胶囊对酒精性肝损伤的防治作用以及增强酒精代谢功效研究。方法各实验组灌胃给予不同浓度受试样品后,建立酒精性肝损伤及急性酒精中毒模型。测定血清中丙氨酸氨基转移酶(ALT)、天门冬氨酸氨基转移酶(AST)、谷氨酰氨基转移酶(GGT)、乙醇和乙醛含量;检测肝脏中三酰甘油(TG)、超氧化物歧化酶(SOD)水平;HE染色观察肝脏和胃组织的病理变化。结果与模型组比较,解酒护肝胶囊中剂量组能显著降低血清中ALT含量(P<0.05),中、高剂量组能显著降低AST和GGT含量(P<0.01),低、中、高剂量组均能显著降低肝脏组织中TG含量(P<0.01),高剂量组显著提高SOD水平(P<0.01)。肝脏组织病理切片显示,模型组大鼠肝细胞大小不均,肝索排列紊乱,有较多小泡性脂肪空泡,同时伴有炎症浸润、细胞坏死,各剂量给药组均可不同程度改善大鼠肝脏组织病变情况;与模型组比较,解酒护肝胶囊高剂量组在醉酒后8 h内乙醇和乙醛含量均显著下降(P<0.01)。解酒护肝胶囊低、高剂量组肝脏和胃组织的病变情况均有所缓解。结论解酒护肝胶囊可抑制氧化应激反应,降低脂质过氧化,加速酒精代谢,对肝组织和胃黏膜具有一定的保护作用。Objective To evaluate the preventive and therapeutic effects of Jiejiu Hugan Capsules on alcoholic liver injury and their ability to enhance alcohol metabolism.Methods After administering different concentrations of test samples by gavage to each experimental group,alcoholic liver injury and acute alcoholism models were established.The levels of serum alanine aminotransferase(ALT),aspartate aminotransferase(AST),glutamyl aminotransferase(GGT),ethanol,and acetaldehyde were measured.The levels of triglycerides(TG)and superoxide dismutase(SOD)in the liver were measured.HE staining was used to observe the pathological changes in liver and gastric tissues.Results Compared with the model group,the medium dose group of Jiejiu Hugan Capsules significantly reduced the serum ALT content(P<0.05),while the medium and high dose groups significantly reduced the AST and GGT content(P<0.01).The low,medium and high dose groups all significantly reduced the TG content in liver tissue(P<0.01),and the medium dose group significantly increased the SOD level(P<0.01).The pathological sections of the liver tissue showed that the liver cells in the model group of rats were uneven in size,with disordered arrangement of liver cords and numerous small vesicular fat vacuoles,accompanied by inflammatory infiltration and cell necrosis.The different dosage groups improved the liver tissue lesions of rats to varying degrees.Compared with the model group,the high dose group of Jiejiu Hugan Capsules showed a significant decrease in ethanol and acetaldehyde content within 8 hours after getting drunk(P<0.01).The pathological changes in liver and stomach tissues were alleviated in both the low and high dose groups of Jiejiu Hugan Capsules.Conclusion Jiejiu Hugan Capsules can inhibit oxidative stress response,reduce lipid peroxidation,accelerate alcohol metabolism,and have a certain protective effect on liver tissue and gastric mucosa.

关 键 词：解酒护肝胶囊 酒精性肝损伤 急性酒精中毒 胃黏膜损伤 保护作用 

分 类 号：R285.5[医药卫生—中药学]