麦门冬汤调控内质网应激大鼠腹膜透析相关性纤维化的抑制作用  

Inhibitory effect of Maimendong Decoction on dialysis-associated peritoneal fibrosis of endoplasmic reticulum stress rats

作　　者：郭宝珠 程锦绣[1] 靳鑫 贺玉桃 孙羡敏 GUO Baozhu;CHENG Jinxiu;JIN Xin;HE Yutao;SUN Xianmin(Department of Nephrology,the First Affiliated Hospital of Hebei North University,Zhangjiakou 075000,China)

机构地区：[1]河北北方学院附属第一医院肾内科,河北张家口075000

出　　处：《广东药科大学学报》2024年第2期57-62,共6页Journal of Guangdong Pharmaceutical University

基　　金：张家口市重点研发计划项目(2221090D);河北省医学科学研究课题(20231411)。

摘　　要：目的研究麦门冬汤调控内质网应激(ERS)大鼠腹膜透析相关性纤维化(PF)的抑制作用。方法将雄性SD大鼠随机分为对照组、模型组和麦门冬汤低、中、高剂量组,采用腹腔注射4.25%葡萄糖腹膜透析液的方式建立腹膜透析相关PF模型,麦门冬汤低、中、高剂量组分别给予8.69、17.37、34.73 g/kg麦门冬汤灌胃,1次/d;对照组和模型组给予生理盐水3 mL灌胃,1次/d,连续4周。评估腹膜功能指标超滤体积和葡萄糖转运量,HE染色观察腹膜组织学变化,Masson染色检测腹膜厚度,荧光定量PCR检测α-平滑肌肌动蛋白(α-SMA)、I型胶原蛋白(Col-I)的mRNA表达水平,Western blot检测α-SMA、Col-I磷酸化蛋白激酶R样内质网激酶(p-PERK)、磷酸化真核翻译起始因子2α(p-eIF2α)、转录激活因子4(ATF4)、C/EBP同源蛋白(CHOP)的蛋白表达水平。结果模型组大鼠的超滤体积低于对照组,葡萄糖转运量、腹膜厚度、α-SMA、Col-I的mRNA和蛋白相对表达水平及p-PERK、p-eIF2α、ATF4、CHOP的蛋白相对表达水平均显著高于对照组(P<0.05);麦门冬汤低、中、高剂量组大鼠的超滤体积高于模型组,葡萄糖转运量、腹膜厚度、α-SMA、Col-I的mRNA和蛋白相对表达水平及p-PERK、p-eIF2α、ATF4、CHOP的蛋白相对表达水平均显著低于模型组(P<0.05)。结论麦门冬汤能改善腹膜透析相关PF,抑制ERS是麦门冬汤发挥PF改善作用的可能分子机制。Objective To study the inhibitory effect of Maimendong Decoction on peritoneal dialysis-associated fibrosis(PF)of endoplasmic reticulum stress(ERS)rats.Methods Male SD rats were randomly divided into control group,model group and low,medium and high dose groups of Maimendong Decoction.Peritoneal dialysation-related PF model was established by intraperitoneal injection of 4.25%glucose dialysate.The low,medium and high dose groups were given 8.69,17.37 and 34.73 g/kg of Maimendong Decoction once a day,the control group and model group were given 3 mL of normal saline once a day for 4 weeks.Peritoneal function indexes were evaluated by ultrafiltration volume and glucose transfer volume.Peritoneal histological changes were observed by HE staining.Peritoneal thickness was detected by Masson staining.The mRNA expression levels ofαsmooth muscle actin(α-SMA)and type I collagen(Col-I)were detected by fluorescence quantitative PCR.The protein expression levels ofα-SMA,Col-I,phosphorylated protein kinase R-like ER kinase(p-PERK),phosphorylated eukaryotic initiation factor2α(p-eIF2α),transcription activating factor 4(ATF4)and C/EBP homologous protein(CHOP)were detected by western blot.Results The ultrafiltration volume of model group was lower than that of the control group,and the glucose transfer volume,peritoneal thickness,the relative mRNA and protein levels ofα-SMA,Col-I,the protein expression of p-PERK,p-eIF2α,ATF4 and CHOP were significantly higher than those of control group(P<0.05).The ultrafiltration volume of low,medium and high dose groups of Maimendong Decoction washigher than that of the model group,and the glucose transfer volume,peritoneal thickness,the relative mRNA and protein expression levels ofα-SMA,Col-I,the protein expression of p-PERK,p-eIF2α,ATF4 and CHOP were significantly lower than those of the model group(P<0.05).Conclusion Maimengdong Decoction can improve peritoneal dialysis-related PF,which is related to inhibition of ERS.

关 键 词：麦门冬汤 腹膜透析 腹膜纤维化 内质网应激 大鼠 

分 类 号：R285.5[医药卫生—中药学]