分子印迹聚合物对血液中野百合碱-血红蛋白加合物的分析  

Analysis of Monocrotaline-hemoglobin adducts in blood using molecularly imprinted polymers

作　　者：葛燕辉 郑远茹 郭景灿 江芷惠 GE Yanhui;ZHENG Yuanru;GUO Jingcan;JIANG Zhihui(School of Pharmacy,Guangdong Pharmaceutical University,Guangzhou 510006,China;The First Affiliated Hospital,Guangdong Pharmaceutical University,Guangzhou 510080,China)

机构地区：[1]广东药科大学药学院,广东广州510006 [2]广东药科大学附属第一医院(临床医学院),广东广州510080

出　　处：《广东药科大学学报》2024年第2期76-82,共7页Journal of Guangdong Pharmaceutical University

基　　金：广州市基础与应用基础研究项目(SL2022A04J00538)。

摘　　要：目的基于分子印迹技术,建立快速检测体内野百合碱和血红蛋白加合物的方法。方法以制备的野百合碱血红蛋白加合物为模板分子,经表面印迹聚合法制备野百合碱-血红蛋白分子印迹聚合物,用作固相萃取介质识别造模大鼠血液中的血红蛋白加合物。结果理化性质表征结果显示聚合层成功接枝在碳纳米管表面,且聚合物具备良好的热稳定性;将聚合物作为固相萃取介质,对造模大鼠血液进行分析,结果显示聚合物血红蛋白加合物具有较强的吸附能力(吸附量达90.86 mg/mg),该结果与肝脏病理检测结果呈正相关性。结论所制备的聚合物可以快速分析体内血红蛋白加合物的含量,与肝脏病理结果相结合,为肝小静脉闭塞症的无创伤诊断提供新的研究思路。Objective To establish a rapid analytical method for in vivo binding of monocrotaline and hemoglobin based on molecular imprinting technology.Methods The prepared monocrotaline hemoglobin adduct(Mct@BHb)was used as a template molecule to prepare molecularly imprinted polymers(Mct@BHb@MIPs)by using surface imprinting polymerization method,and then the Mct@BHb@MIPs were used as an extraction medium to adsorp toxic markers in rat blood.Results The results showed that the polymer layer was successfully grafted onto the surface of MWCNs,and the polymer exhibited good thermal stability after characterization.The Mct@BHb@MIPs were used as a solid-phase extraction medium to analyze the blood of model rats,and the results showed that the polymer hemoglobin adducts had good adsorption capacity(adsorption amount reached 90.86 mg/mg).The analysis results were consistent with liver pathological detection.Conclusion The polymer prepared by this research can quickly analyze the content of hemoglobin conjugates in vivo,which provides new research ideas for non-invasive diagnosis of hepatic small vein occlusion,combined with pathological results.

关 键 词：野百合碱 血红蛋白加合物 分子印迹聚合物 肝小静脉闭塞症 无创诊断 

分 类 号：R917[医药卫生—药物分析学]