Biomimetic design of highly flexible metal oxide nanofibrous membranes with exceptional mechanical performance for superior phosphopeptide enrichment  

作　　者：Xue Mao Zhen-Zhen Li Dong-Lian Hao Wei-Dong Han Gao-Peng Li Yao-Yu Wang Kun Zhang 

机构地区：[1]School of Textile Science and Engineering,Key Laboratory of Functional Textile Material and Product(Ministry of Education),Xi’an Polytechnic University,Xi’an 710048,China [2]Key Laboratory of Magnetic Molecules&Magnetic Information Materials of the Ministry of Education,School of Chemistry&Material Science,Shanxi Normal University,Taiyuan 030031,China [3]Department of Clinical Laboratory,The Second Affiliated Hospital of Xi’an Jiaotong University,Xi’an 710004,China [4]Shanghai iProteome Biotechnology Co.,Ltd,Shanghai 201210,China [5]College of Chemistry&Materials Science,Northwest University,Xi'an 710127,China

出　　处：《Nano Research》2024年第5期4270-4278,共9页纳米研究（英文版）

基　　金：supported by the National Natural Science Foundation of China(Nos.52202110,22201167);the Natural Science Foundation of Science and Technology Agency of Shanxi Province(No.20210302124654);the Scientific and Technological Innovation Programs of Higher Education Institutions in Shanxi(No.2021L259);the Innovation and Entrepreneurship Training Program for College students in Shanxi Province(No.20220312);the Outstanding Young Talents of Shaanxi Universities(2019);the Scientific and Technological Plan Project of Xi’an(No.21XJZZ0012);the Key Research and Development Program of Shaanxi Province of China(No.2022SF-201);the Service Local Special Program of Education Department of Shaanxi Province(No.23JC029);the Scientific and Technological Plan Project of the Beilin District of Xi’an City(No.GX2206).

摘　　要：Developing free-standing and mechanical robust membrane materials capable of superior enrichment of phosphopeptides for analyzing and identifying the specific phosphoproteome of cancer cells is significant in understanding the molecular mechanisms of cancer development and exploring new therapeutic approaches,but still a significant challenge in materials design.To this end,we firstly constructed highly flexible ZrTiO_(4) nanofibrous membranes(NFMs)with excellent mechanical stability through a cost-effective and scalable electrospinning and subsequent calcination technique.Then,to further increase the enrichment capacity of the phosphopeptide,the biomimetic TiO_(2)@ZrTiO_(4) NFMs with root hair or leaf like branch microstructure are developed by the hydrothermal post-synthetic modification of ZrTiO_(4) NFMs through growing unfurling TiO_(2) nanosheets onto the ZrTiO_(4) nanofibers.Importantly,remarkable flexibility and mechanical stability enable the resulting TiO_(2)@ZrTiO_(4) NFMs excellent practicability,while the biomimetic microstructure allows it outstanding enrichment ability of the phosphopeptide and identification ability of the specific phosphoproteins in the digest of cervical cancer cells.Specifically,6770 phosphopeptides can be enriched by TiO_(2)@ZrTiO_(4) NFMs(2205 corresponding phosphoproteins can be identified),and the value is much higher than that of ZrTiO_(4) NFMs(6399 phosphopeptides and 2132 identified phosphoproteins)and commercial high-performance TiO_(2) particles(4525 phosphopeptides and 1811 identified phosphoproteins).These results demonstrate the super ability of TiO_(2)@ZrTiO_(4) NFMs in phosphopeptide enrichment and great potential for exploring the pathogenesis of cancer.

关 键 词：inorganic nanofibers flexible ceramic nanofibrous membranes biomimetic materials exceptional mechanical performance FLEXIBILITY 

分 类 号：TB383[一般工业技术—材料科学与工程]